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https://hdl.handle.net/2440/76681
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dc.contributor.author | Matthews, G. | - |
dc.contributor.author | Howarth, G. | - |
dc.contributor.author | Butler, R. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Chemotherapy: international journal of experimental and clinical chemotherapy, 2012; 58(2):102-109 | - |
dc.identifier.issn | 0009-3157 | - |
dc.identifier.issn | 1421-9794 | - |
dc.identifier.uri | http://hdl.handle.net/2440/76681 | - |
dc.description.abstract | BACKGROUND: Short-chain fatty acids (SCFA) are undergoing increased scrutiny as chemotherapeutics for colon cancer, although a comprehensive understanding of their mode of action is lacking. We investigated candidate SCFA for their capability to modulate apoptosis, cell cycle, intracellular redox state and glucose metabolism in the Caco-2 human colon cancer cell line. METHODS: Caco-2 cells were incubated with butyrate, propionate or a combination of these SCFA (1:1) and assessed by flow cytometry, enzyme activity analysis or by isotope ratio mass spectrometry. RESULTS: Butyrate and the SCFA combination induced apoptosis and G2-M arrest to a greater extent than propionate alone (p < 0.05). SCFA treatment led to time-dependent alterations to the oxidative pentose pathway, reductions in glutathione availability and increases in levels of reactive oxygen species (p < 0.05) compared with untreated controls. The rate of D-glucose metabolism was increased by all SCFA, although to the greatest extent by butyrate (p < 0.05). CONCLUSIONS: These results suggest that butyrate, or the combination of both SCFA, induced rapid and extensive apoptosis and G2-M arrest associated with changes to redox state and D-glucose metabolism. These results support the potential for butyrate and propionate to act as adjuncts to conventional chemotherapy regimens for colon cancer. | - |
dc.description.statementofresponsibility | Matthews G.M., Howarth G.S., Butler R.N. | - |
dc.language.iso | en | - |
dc.publisher | Karger | - |
dc.rights | Copyright © 2012 S. Karger AG, Basel | - |
dc.source.uri | http://dx.doi.org/10.1159/000335672 | - |
dc.subject | Butyrate | - |
dc.subject | Propionate | - |
dc.subject | Short-chain fatty acid | - |
dc.subject | Colon cancer | - |
dc.subject | Apoptosis | - |
dc.subject | Cell cycle | - |
dc.subject | Redox | - |
dc.subject | Glucose metabolism | - |
dc.title | Short-chain fatty acids induce apoptosis in colon cancer cells associated with changes to intracellular redox state and glucose metabolism | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1159/000335672 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Howarth, G. [0000-0001-6979-6084] | - |
Appears in Collections: | Animal and Veterinary Sciences publications Aurora harvest |
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