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Type: Journal article
Title: Molecular characterization, pharmacological properties and chromosomal localization of the human GALR2 galanin receptor
Author: Fathi, Z.
Battaglino, P.
Iben, L.
Li, H.
Baker, E.
Zhang, D.
McGovern, R.
Mahle, C.
Sutherland, G.
Iismaa, T.
Dickinson, K.
Antal Zimanyi, I.
Citation: Molecular Brain Research, 1998; 58(37653):156-169
Issue Date: 1998
ISSN: 0169-328X
Statement of
Fathi, Zahra ; Battaglino, Peter M ; Iben, Lawrence G ; Li, Hui ; Baker, Elizabeth ; Zhang, Donglu ; McGovern, Rachel ; Mahle, Cathy D ; Sutherland, Grant R ; Iismaa, Tiina P ; Dickinson, Kenneth E.J ; Antal Zimanyi, Ildiko
Abstract: The neuropeptide galanin mediates a diverse spectrum of biological activities by interacting with specific G protein-coupled receptors. We have used homology genomic library screening and polymerase chain reaction (PCR) techniques to isolate both genomic and cDNA clones encoding the human homolog of the recently cloned rat GALR2 galanin receptor. By fluorescence in situ hybridization, the gene encoding human GALR2 (GALNR2) has been localized to chromosome 17q25.3. The two coding exons of the human GALNR2 gene, interrupted by an intron positioned at the end of transmembrane domain III, encode a 387 amino acid G protein-coupled receptor with 87% overall amino acid identity with rat GALR2. In HEK-293 cells stably expressing human GALR2, binding of [125I]porcine galanin is saturable and can be displaced by galanin, amino-terminal galanin fragments and chimeric galanin peptides but not by carboxy-terminal galanin fragments. In HEK-293 cells, human GALR2 couples both to Galphaq/11 to stimulate phospholipase C and increase intracellular calcium levels and to Galphai/o to inhibit forskolin-stimulated intracellular cAMP accumulation. A wide tissue distribution is observed by reverse transcriptase (RT)-PCR analysis, with human GALR2 mRNA being detected in many areas of the human central nervous system as well as in peripheral tissues.
Keywords: Cell Line; Chromosomes, Human, Pair 17; Animals; Swine; Humans; Rats; GTP-Binding Proteins; Phosphatidylinositols; Galanin; Receptors, Galanin; Receptors, Neuropeptide; Recombinant Proteins; DNA, Complementary; In Situ Hybridization, Fluorescence; Chromosome Mapping; Cloning, Organism; Transfection; Sequence Alignment; Amino Acid Sequence; Base Sequence; Sequence Homology, Amino Acid; Kinetics; Molecular Sequence Data
RMID: 0030005445
DOI: 10.1016/S0169-328X(98)00116-8
Appears in Collections:Paediatrics publications

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