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Type: Journal article
Title: The brn-2 gene regulates the melanocytic phenotype and tumorigenic potential of human melanoma cells
Author: Angus, J.
Thompson, F.
Murphy, K.
Baker, E.
Sutherland, G.
Parsons, P.
Sturm, R.
Citation: Oncogene, 1995; 11(4):691-700
Publisher: Macmillan Press
Issue Date: 1995
ISSN: 0950-9232
Abstract: The Oct transcription factors N-Oct-3 and N-Oct-5 are differentially expressed in normal melanocytes, melanoma tumors and cell lines. We have cloned the human brn-2 gene and have shown that it encodes both the N-Oct-3 and N-Oct-5 octamer binding activities detected in melanoma cells. The brn-2 genomic locus has been mapped to chromosome 6q16 and although chromosomal aberrations are common in this region in melanoma, no deletion or rearrangement of the brn-2 gene in melanoma cell lines was observed. Sequencing of the entire gene showed that there are no intervening sequences within the open reading frame. Antisense RNA-mediated inhibition of brn-2 gene expression in melanoma cells was associated with a change in morphology and loss of melanocytic and neural crest markers, including the melanocyte transcription factor microphthalmia and the TYRP pigmentation genes. In addition, loss of brn-2 in these cells resulted in the complete loss of ability to form tumors in SCID and nu/nu mice. These results suggest roles for brn-2 in the determination of the melanocytic lineage and in the tumorigenic phenotype of melanoma.
Keywords: Cell Line; Tumor Cells, Cultured; Chromosomes, Human, Pair 6; Animals; Humans; Mice; Mice, SCID; Melanoma; Chromosome Deletion; Homeodomain Proteins; Intercellular Adhesion Molecule-1; Transcription Factors; RNA, Antisense; DNA, Neoplasm; Blotting, Southern; In Situ Hybridization, Fluorescence; Restriction Mapping; Transfection; Cell Differentiation; Gene Expression; Gene Expression Regulation, Neoplastic; Base Sequence; Molecular Sequence Data; POU Domain Factors
RMID: 0030005417
Appears in Collections:Paediatrics publications

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