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Type: Journal article
Title: Isolation and characterization of mesenchymal stem cell-like cells from healthy and inflamed gingival tissue: Potential use for clinical therapy
Author: Ge, S.
Mrozik, K.
Menicanin, D.
Gronthos, S.
Bartold, P.
Citation: Regenerative Medicine, 2012; 7(6):819-832
Publisher: Future Medicine Ltd
Issue Date: 2012
ISSN: 1746-0751
Statement of
Shaohua Ge, Krzysztof Marek Mrozik, Danijela Menicanin, Stan Gronthos, & P Mark Bartold
Abstract: Aim: Postnatal mesenchymal stem cell (MSC)-like cells have previously been isolated and ex vivo-expanded from healthy gingival tissues. The aim of this research was to isolate and characterize MSC-like cells from inflamed gingival tissues and determine whether they retain the characteristics of MSC-like cells from healthy gingival tissues. Materials & methods: Fifteen clonal lines of MSC-like cells from three healthy gingival tissues (GMSC-H) and fifteen from three inflamed gingival tissues (GMSC-I) were generated. Bulk-cultured cell lines from healthy and inflamed gingival tissues were also established. In vitro and in vivo characterization studies of GMSC-Is were performed relative to GMSC-Hs. Results: The incidence of clonogenic colony forming units-fibroblast was comparable between healthy and inflamed gingival tissues. GMSC-H and GMSC-I clones expressed MSC-associated markers CD44, CD73, CD90, CD105 and CD166. While the population doubling capacity of GMSC-Is was reduced compared with GMSC-Hs, both populations displayed a similar capacity to undergo osteogenic, adipogenic and chondrogenic differentiation in vitro. Following subcutaneous implantation in NOD/SCID mice, both GMSC-Hs and GMSC-Is formed dense connective tissue-like structures in vivo resembling natural gingival tissue. Conclusion: MSC-like populations exist within inflamed gingival tissue that are functionally equivalent to MSC-like cells derived from healthy gingival tissue. Given the relative abundance of inflamed gingival tissue and ease of accessibility, MSC-like cells from inflamed gingival tissues represent a newly identified population of postnatal stem cells with immense potential in tissue engineering applications.
Keywords: differentiation; gingival; inflammation; mesenchymal stem cells; tissue engineering
Rights: Copyright status unknown
RMID: 0020123183
DOI: 10.2217/rme.12.61
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Appears in Collections:Dentistry publications

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