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|Title:||Isolation and characterization of mesenchymal stem cell-like cells from healthy and inflamed gingival tissue: Potential use for clinical therapy|
|Citation:||Regenerative Medicine, 2012; 7(6):819-832|
|Publisher:||Future Medicine Ltd|
|Shaohua Ge, Krzysztof Marek Mrozik, Danijela Menicanin, Stan Gronthos, & P Mark Bartold|
|Abstract:||Aim: Postnatal mesenchymal stem cell (MSC)-like cells have previously been isolated and ex vivo-expanded from healthy gingival tissues. The aim of this research was to isolate and characterize MSC-like cells from inflamed gingival tissues and determine whether they retain the characteristics of MSC-like cells from healthy gingival tissues. Materials & methods: Fifteen clonal lines of MSC-like cells from three healthy gingival tissues (GMSC-H) and fifteen from three inflamed gingival tissues (GMSC-I) were generated. Bulk-cultured cell lines from healthy and inflamed gingival tissues were also established. In vitro and in vivo characterization studies of GMSC-Is were performed relative to GMSC-Hs. Results: The incidence of clonogenic colony forming units-fibroblast was comparable between healthy and inflamed gingival tissues. GMSC-H and GMSC-I clones expressed MSC-associated markers CD44, CD73, CD90, CD105 and CD166. While the population doubling capacity of GMSC-Is was reduced compared with GMSC-Hs, both populations displayed a similar capacity to undergo osteogenic, adipogenic and chondrogenic differentiation in vitro. Following subcutaneous implantation in NOD/SCID mice, both GMSC-Hs and GMSC-Is formed dense connective tissue-like structures in vivo resembling natural gingival tissue. Conclusion: MSC-like populations exist within inflamed gingival tissue that are functionally equivalent to MSC-like cells derived from healthy gingival tissue. Given the relative abundance of inflamed gingival tissue and ease of accessibility, MSC-like cells from inflamed gingival tissues represent a newly identified population of postnatal stem cells with immense potential in tissue engineering applications.|
|Keywords:||differentiation; gingival; inflammation; mesenchymal stem cells; tissue engineering|
|Rights:||Copyright status unknown|
|Appears in Collections:||Dentistry publications|
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