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https://hdl.handle.net/2440/77641
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dc.contributor.author | Ge, S. | - |
dc.contributor.author | Mrozik, K. | - |
dc.contributor.author | Menicanin, D. | - |
dc.contributor.author | Gronthos, S. | - |
dc.contributor.author | Bartold, P. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | Regenerative Medicine, 2012; 7(6):819-832 | - |
dc.identifier.issn | 1746-0751 | - |
dc.identifier.issn | 1746-076X | - |
dc.identifier.uri | http://hdl.handle.net/2440/77641 | - |
dc.description.abstract | Aim: Postnatal mesenchymal stem cell (MSC)-like cells have previously been isolated and ex vivo-expanded from healthy gingival tissues. The aim of this research was to isolate and characterize MSC-like cells from inflamed gingival tissues and determine whether they retain the characteristics of MSC-like cells from healthy gingival tissues. Materials & methods: Fifteen clonal lines of MSC-like cells from three healthy gingival tissues (GMSC-H) and fifteen from three inflamed gingival tissues (GMSC-I) were generated. Bulk-cultured cell lines from healthy and inflamed gingival tissues were also established. In vitro and in vivo characterization studies of GMSC-Is were performed relative to GMSC-Hs. Results: The incidence of clonogenic colony forming units-fibroblast was comparable between healthy and inflamed gingival tissues. GMSC-H and GMSC-I clones expressed MSC-associated markers CD44, CD73, CD90, CD105 and CD166. While the population doubling capacity of GMSC-Is was reduced compared with GMSC-Hs, both populations displayed a similar capacity to undergo osteogenic, adipogenic and chondrogenic differentiation in vitro. Following subcutaneous implantation in NOD/SCID mice, both GMSC-Hs and GMSC-Is formed dense connective tissue-like structures in vivo resembling natural gingival tissue. Conclusion: MSC-like populations exist within inflamed gingival tissue that are functionally equivalent to MSC-like cells derived from healthy gingival tissue. Given the relative abundance of inflamed gingival tissue and ease of accessibility, MSC-like cells from inflamed gingival tissues represent a newly identified population of postnatal stem cells with immense potential in tissue engineering applications. | - |
dc.description.statementofresponsibility | Shaohua Ge, Krzysztof Marek Mrozik, Danijela Menicanin, Stan Gronthos, & P Mark Bartold | - |
dc.language.iso | en | - |
dc.publisher | Future Medicine Ltd | - |
dc.rights | Copyright status unknown | - |
dc.source.uri | http://dx.doi.org/10.2217/rme.12.61 | - |
dc.subject | differentiation | - |
dc.subject | gingival | - |
dc.subject | inflammation | - |
dc.subject | mesenchymal stem cells | - |
dc.subject | tissue engineering | - |
dc.title | Isolation and characterization of mesenchymal stem cell-like cells from healthy and inflamed gingival tissue: Potential use for clinical therapy | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.2217/rme.12.61 | - |
dc.relation.grant | http://purl.org/au-research/grants/nhmrc/627143 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Mrozik, K. [0000-0002-4890-8208] | - |
dc.identifier.orcid | Menicanin, D. [0000-0002-1178-2293] | - |
dc.identifier.orcid | Gronthos, S. [0000-0002-6225-3084] | - |
dc.identifier.orcid | Bartold, P. [0000-0002-5695-3877] | - |
Appears in Collections: | Aurora harvest Dentistry publications |
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