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Type: Journal article
Title: Evaluation of pre-analysis loss of dependent drugs in wastewater: stability and binding assessments
Author: Chen, C.
Kostakis, C.
Irvine, R.
Felgate, P.
White, J.
Citation: Drug Testing and Analysis, 2013; 5(8):716-721
Publisher: John Wiley & Sons Ltd
Issue Date: 2013
ISSN: 1942-7611
Statement of
Chang Chen, Chris Kostakis, Rodney J. Irvine, Peter D. Felgate and Jason M. White
Abstract: Wastewater analysis has the potential to provide objective and timely data on population drug consumption, but some crucial factors such as pre-analysis drug loss during sample storage and filtration could affect the accuracy and reliability of the method, and these uncertainties have yet to be fully assessed. This study was designed to evaluate analyte stability in wastewater stored under different conditions with the aim of optimizing the sample storage procedures for future studies. It also investigated whether there is significant analyte loss during filtration before sample extraction and storage after that. The studied substances and metabolites were: cotinine, cocaine and its metabolite benzoylecgonine, phenethylamines amphetamine, methamphetamine, 3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), opioids including codeine, methadone, 6-monoacetylmorphine (MAM) and morphine. In most situations, storing samples at 4 °C is sufficient to stabilize analytes for at least 2 weeks, and refrigeration is unnecessary during sample transportation within 3 days. However, additional measures need to be taken if unstable analytes such as cocaine and MAM are to be analyzed. No significant analyte loss was observed in the filtration process or in reconstituted extract stored at 4 °C or -20 °C for 2 weeks. By choosing stable analytes and proper storage conditions, wastewater analysis has the potential to provide accurate data for estimation of community drug use.
Keywords: Amphetamine; Methamphetamine; N-Methyl-3,4-methylenedioxyamphetamine; Morphine; Morphine Derivatives; Codeine; Cocaine; Cotinine; Street Drugs; Water Pollutants, Chemical; Chromatography, Liquid; Drug Stability; Tandem Mass Spectrometry; Liquid-Liquid Extraction; Waste Water
Description: Article first published online: 9 OCT 2012
Rights: Copyright © 2012 John Wiley & Sons, Ltd.
RMID: 0020127112
DOI: 10.1002/dta.1428
Appears in Collections:Pharmacology publications

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