Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Type: Journal article
Title: Dasatinib targets chronic myeloid leukemia-CD34⁺ progenitors as effectively as it targets mature cells
Other Titles: Dasatinib targets chronic myeloid leukemia-CD34(+) progenitors as effectively as it targets mature cells
Author: Hiwase, D.
Saunders, V.
Nievergall, E.
Ross, D.
White, D.
Hughes, T.
Citation: Haematologica, 2013; 98(6):896-900
Publisher: Ferrata Storti Foundation
Issue Date: 2013
ISSN: 0390-6078
Statement of
Devendra K. Hiwase, Verity A. Saunders, Eva Nievergall, Douglas D. Ross, Deborah L. White, and Timothy P. Hughes
Abstract: Dasatinib is effective in most chronic phase chronic myeloid leukemia patients both in first-line therapy and following imatinib failure. While imatinib uptake into CD34(+) cells is low compared to mononuclear cells, few data evaluate how well dasatinib targets primitive CML cells. This study compares intracellular concentration of dasatinib and Bcr-Abl kinase inhibition in CML-CD34(+) progenitors and mononuclear cells induced by dasatinib. The intracellular concentrations of dasatinib were similar between CML-CD34(+) and mononuclear cells (P=0.8). Similarly, there was no significant difference in the degree of dasatinib-mediated Bcr-Abl kinase inhibition. ABCB1 (MDR1) and ABCG2 inhibitors neither increased dasatinib intracellular concentration nor enhanced dasatinib-mediated Bcr-Abl kinase inhibition. In contrast to nilotinib, we show that dasatinib is not an ABCB1 inhibitor. Thus, dasatinib targets CML-CD34(+) progenitors as effectively as it targets mononuclear cells. ABCB1 and ABCG2 efflux pumps do not appear to influence the intracellular dasatinib concentration in CML-CD34(+) progenitors.
Keywords: Leukocytes, Mononuclear; Cells, Cultured; Humans; Pyrimidines; Thiazoles; ATP-Binding Cassette Transporters; Neoplasm Proteins; Fusion Proteins, bcr-abl; Antineoplastic Agents; Antigens, CD34; Protein Kinase Inhibitors; Substrate Specificity; Neoplastic Stem Cells; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Dasatinib; ATP Binding Cassette Transporter, Sub-Family G, Member 2; ATP Binding Cassette Transporter, Sub-Family B; ATP-Binding Cassette, Sub-Family B, Member 1
Rights: ©2013 Ferrata Storti Foundation
RMID: 0020128840
DOI: 10.3324/haematol.2012.070268
Appears in Collections:Medicine publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.