Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/79026
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Type: | Journal article |
Title: | Viperin is induced following dengue virus type-2 (DENV-2) infection and has anti-viral actions requiring the C-terminal end of viperin |
Author: | Helbig, K. Carr, J. Calvert, J. Wati, S. Clarke, J. Eyre, N. Narayana, S. Fiches, G. McCartney, E. Beard, M. |
Citation: | PLoS Neglected Tropical Diseases, 2013; 7(4):1-14 |
Publisher: | Public Library of Science |
Issue Date: | 2013 |
ISSN: | 1935-2727 1935-2735 |
Editor: | Michael, S.F. |
Statement of Responsibility: | Karla J. Helbig, Jillian M. Carr, Julie K. Calvert, Satiya Wati, Jennifer N. Clarke, Nicholas S. Eyre, Sumudu K. Narayana, Guillaume N. Fiches, Erin M. McCartney, Michael R. Beard |
Abstract: | The host protein viperin is an interferon stimulated gene (ISG) that is up-regulated during a number of viral infections. In this study we have shown that dengue virus type-2 (DENV-2) infection significantly induced viperin, co-incident with production of viral RNA and via a mechanism requiring retinoic acid-inducible gene I (RIG-I). Viperin did not inhibit DENV-2 entry but DENV-2 RNA and infectious virus release was inhibited in viperin expressing cells. Conversely, DENV-2 replicated to higher tires earlier in viperin shRNA expressing cells. The anti-DENV effect of viperin was mediated by residues within the C-terminal 17 amino acids of viperin and did not require the N-terminal residues, including the helix domain, leucine zipper and S-adenosylmethionine (SAM) motifs known to be involved in viperin intracellular membrane association. Viperin showed co-localisation with lipid droplet markers, and was co-localised and interacted with DENV-2 capsid (CA), NS3 and viral RNA. The ability of viperin to interact with DENV-2 NS3 was associated with its anti-viral activity, while co-localisation of viperin with lipid droplets was not. Thus, DENV-2 infection induces viperin which has anti-viral properties residing in the C-terminal region of the protein that act to restrict early DENV-2 RNA production/accumulation, potentially via interaction of viperin with DENV-2 NS3 and replication complexes. These anti-DENV-2 actions of viperin show both contrasts and similarities with other described anti-viral mechanisms of viperin action and highlight the diverse nature of this unique anti-viral host protein. |
Keywords: | Cell Line, Tumor Vero Cells Animals Humans Dengue Virus Dengue Proteins Blotting, Western Reverse Transcriptase Polymerase Chain Reaction Chlorocebus aethiops |
Rights: | © 2013 Helbig et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pntd.0002178 |
Published version: | http://dx.doi.org/10.1371/journal.pntd.0002178 |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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File | Description | Size | Format | |
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hdl_79026.pdf | Published version | 7.24 MB | Adobe PDF | View/Open |
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