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https://hdl.handle.net/2440/79561
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Type: | Journal article |
Title: | A reporter system for replication-competent gammaretroviruses: the inGluc-MLV-DERSE assay |
Author: | Aloia, A. Duffy, L. Pak, V. Lee, K. Sanchez-Martinez, S. Derse, D. Heidecker, G. Cornetta, K. Rein, A. |
Citation: | Gene Therapy (Basingstoke), 2013; 20(2):169-176 |
Publisher: | Nature Publishing Group |
Issue Date: | 2013 |
ISSN: | 0969-7128 1476-5462 |
Statement of Responsibility: | AL Aloia, L Duffy, V Pak, KE Lee, S Sanchez-Martinez, D Derse, G Heidecker, K Cornetta, and A Rein |
Abstract: | Although novel retroviral vectors for use in gene-therapy products are reducing the potential for formation of replication-competent retrovirus (RCR), it remains crucial to screen products for RCR for both research and clinical purposes. For clinical-grade gammaretrovirus-based vectors, RCR screening is achieved by an extended S+L− or marker-rescue assay, whereas standard methods for replication-competent lentivirus detection are still in development. In this report, we describe a rapid and sensitive method for replication-competent gammaretrovirus detection. We used this assay to detect three members of the gammaretrovirus family and compared the sensitivity of our assay with well-established methods for retrovirus detection, including the extended S+L− assay. Results presented here demonstrate that this assay should be useful for gene-therapy product testing. |
Keywords: | replication-competent gammaretrovirus gene-therapy vectors infectivity assay |
Rights: | © 2013 Macmillan Publishers Limited |
DOI: | 10.1038/gt.2012.18 |
Published version: | http://dx.doi.org/10.1038/gt.2012.18 |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
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