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Type: Journal article
Title: A tudor domain protein SPINDLIN1 interacts with the mRNA-binding protein SERBP1 and is involved in mouse oocyte meiotic resumption
Author: Chew, T.
Peaston, A.
Lim, A.
Lorthongpanich, C.
Knowles, B.
Solter, D.
Citation: PLoS One, 2013; 8(7):1-10
Publisher: Public Library of Science
Issue Date: 2013
ISSN: 1932-6203
Editor: Sun, Q.-Y.
Statement of
Ting Gang Chew, Anne Peaston, Ai Khim Lim, Chanchao Lorthongpanich, Barbara B. Knowles, Davor Solter
Abstract: Mammalian oocytes are arrested at prophase I of meiosis, and resume meiosis prior to ovulation. Coordination of meiotic arrest and resumption is partly dependent on the post-transcriptional regulation of maternal transcripts. Here, we report that, SPINDLIN1 (SPIN1), a maternal protein containing Tudor-like domains, interacts with a known mRNA-binding protein SERBP1, and is involved in regulating maternal transcripts to control meiotic resumption. Mouse oocytes deficient for Spin1 undergo normal folliculogenesis, but are defective in resuming meiosis. SPIN1, via its Tudor-like domain, forms a ribonucleoprotein complex with SERBP1, and regulating mRNA stability and/or translation. The mRNA for the cAMP-degrading enzyme, PDE3A, is reduced in Spin1 mutant oocytes, possibly contributing to meiotic arrest. Our study demonstrates that Spin1 regulates maternal transcripts post-transcriptionally and is involved in meiotic resumption.
Keywords: Ovarian Follicle
Hyaluronic Acid
RNA-Binding Proteins
Cell Cycle Proteins
Myogenic Regulatory Factors
Microtubule-Associated Proteins
RNA, Messenger
Mutagenesis, Site-Directed
Gene Expression Regulation
Amino Acid Sequence
Protein Structure, Tertiary
Protein Binding
Models, Molecular
Molecular Sequence Data
Rights: © 2013 Chew et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0069764
Published version:
Appears in Collections:Animal and Veterinary Sciences publications
Aurora harvest

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