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|dc.identifier.citation||Immunology and Cell Biology, 1997; 75(5):508-510||en|
|dc.description.abstract||The 5' flanking region of human factor H was cloned using nested polymerase chain reaction (PCR) and the promoter finder method. A total of 1.2 kb has been sequenced and a number of putative regulatory elements identified including glucocorticoid response elements, cAMP responsive element, HTF-1, and acute phase signal sequences. A 717 b.p. fragment was cloned into a CAT reporter vector and transfected into HeLa cells. A series of truncations from the 5' end of this fragment were also cloned into the CAT vector. Analysis of CAT activity of the cell lysates showed that the region from -699 to +18 is likely to contain promoter elements for the factor H gene as it was able to drive transcription of the CAT gene.||en|
|dc.description.statementofresponsibility||Helena M Ward, Naomi H Higgs, Timothy K Blackmore, Tania A Sadlon and David L Gordon||en|
|dc.publisher||Nature Publishing Group||en|
|dc.rights||Copyright status unknown||en|
|dc.subject||Cloning; Complement; Gene promoter||en|
|dc.title||Cloning and analysis of the human complement factor H gene promoter||en|
|pubs.library.collection||Medical Education Unit publications||en|
|dc.identifier.orcid||Ward, H. [0000-0002-3831-1205]||en|
|Appears in Collections:||Medical Education Unit publications|
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