Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/82022
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dc.contributor.authorSugimura, S.en
dc.contributor.authorRitter, L.en
dc.contributor.authorSutton-McDowall, M.en
dc.contributor.authorMottershead, D.en
dc.contributor.authorThompson, J.en
dc.contributor.authorGilchrist, R.en
dc.date.issued2014en
dc.identifier.citationMolecular Human Reproduction, 2014; 20(6):499-513en
dc.identifier.issn1460-2407en
dc.identifier.issn1460-2407en
dc.identifier.urihttp://hdl.handle.net/2440/82022-
dc.description.abstractThis study assessed the participation of amphiregulin (AREG) and bone morphogenetic protein 15 (BMP15) during maturation of bovine cumulus oocyte complexes (COCs) on cumulus cell function and their impact on subsequent embryo development. AREG treatment of COCs enhanced blastocyst formation and quality only when in the presence of BMP15. Expression of hyaluronan synthase 2 was enhanced by follicle stimulating hormone (FSH) but not by AREG, which was reflected in the level of cumulus expansion. Although both FSH and AREG stimulated glycolysis, AREG treated COCs had higher glucose consumption, lactate production and ratio of lactate production to glucose uptake. Autofluorescence levels in oocytes, indicative of NAD(P)H and FAD++, were increased with combined AREG and BMP15 treatment of COCs. In contrast, these treatments did not alter autoflouresence levels when cumulus cells were removed from oocytes, even in the presence of other COCs, suggesting oocyte-cumulus gap-junctional communication (GJC) is required. FSH contributed to maintaining GJC for an extended period of time. Remarkably, BMP15 was equally effective at maintaining GJC even in the presence of AREG. Hence, AREG stimulation of COC glycolysis and BMP15 preservation of GJC may facilitate efficient transfer of metabolites from cumulus cells to the oocyte thereby enhancing oocyte developmental competence. These results have implications for improving in vitro oocyte maturation systems.en
dc.description.statementofresponsibilitySatoshi Sugimura, Lesley J Ritter, Melanie L Sutton-McDowall, David G Mottershead, Jeremy G Thompson and Robert B Gilchristen
dc.language.isoenen
dc.publisherOxford University Pressen
dc.rights© The Author 2014. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en
dc.source.urihttp://molehr.oxfordjournals.org/content/early/2014/02/19/molehr.gau013en
dc.subjectAmphiregulin; BMP15; Cumulus cell; Gap-junction; Metabolism; Oocyteen
dc.titleAmphiregulin cooperates with bone morphogenetic protein 15 to increase oocyte developmental competence by gap junction-mediated enhanced metabolite supplyen
dc.typeJournal articleen
dc.contributor.departmentFaculty of Health Sciencesen
dc.identifier.rmid0030000162en
dc.contributor.organisationRobinson Instituteen
dc.identifier.doi10.1093/molehr/gau013en
dc.identifier.pubid64168-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidRitter, L. [0000-0001-5942-851X]en
dc.identifier.orcidSutton-McDowall, M. [0000-0002-4121-0202]en
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]en
dc.identifier.orcidGilchrist, R. [0000-0003-1611-7142]en
Appears in Collections:Obstetrics and Gynaecology publications

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