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Type: Journal article
Title: Induction of apoptosis by the medium-chain length fatty acid lauric acid in colon cancer cells due to induction of oxidative stress
Author: Fauser, J.
Matthews, G.
Cummins, A.
Howarth, G.
Citation: Chemotherapy, 2014; 59(3):214-224
Publisher: Karger
Issue Date: 2014
ISSN: 0009-3157
Statement of
J.K. Fauser, G.M. Matthews, A.G. Cummins, G.S. Howarth
Abstract: BACKGROUND: Fatty acids are classified as short-chain (SCFA), medium-chain (MCFA) or long-chain and hold promise as adjunctive chemotherapeutic agents for the treatment of colorectal cancer. The antineoplastic potential of MCFA remains underexplored; accordingly, we compared the MCFA lauric acid (C12:0) to the SCFA butyrate (C4:0) in terms of their capacity to induce apoptosis, modify glutathione (GSH) levels, generate reactive oxygen species (ROS), and modify phases of the cell cycle in Caco-2 and IEC-6 intestinal cell lines. METHODS: Caco-2 and IEC-6 cells were treated with lauric acid, butyrate, or vehicle controls. Apoptosis, ROS, and cell cycle analysis were determined by flow cytometry. GSH availability was assessed by enzymology. RESULTS: Lauric acid induced apoptosis in Caco-2 (p < 0.05) and IEC-6 cells (p < 0.05) compared to butyrate. In Caco-2 cells, lauric acid reduced GSH availability and generated ROS compared to butyrate (p < 0.05). Lauric acid reduced Caco-2 and IEC-6 cells in G0/G1and arrested cells in the S and G2/M phases. Lauric acid induced apoptosis in IEC-6 cells compared to butyrate (p < 0.05). Butyrate protected IEC-6 cells from ROS-induced damage, whereas lauric acid induced high levels of ROS compared to butyrate. CONCLUSION: Compared to butyrate, lauric acid displayed preferential antineoplastic properties, including induction of apoptosis in a CRC cell line.
Keywords: Medium-chain fatty acid; Short-chain fatty acid; Colorectal cancer; Caco-2; IEC-6; Apoptosis; Redox; Cell cycle; Glutathione; Reactive oxygen species
Rights: © 2013 S. Karger AG, Basel
RMID: 0020134628
DOI: 10.1159/000356067
Appears in Collections:Animal and Veterinary Sciences publications

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