Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/8480
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dc.contributor.authorHarvey, A.en
dc.contributor.authorKind, K.en
dc.contributor.authorThompson, J.en
dc.date.issued2004en
dc.identifier.citationReproduction Fertility and Development, 2004; 16(7):665-673en
dc.identifier.issn1031-3613en
dc.identifier.issn1448-5990en
dc.identifier.urihttp://hdl.handle.net/2440/8480-
dc.description.abstractIn cattle embryos, development to the blastocyst stage is improved in the presence of 10 μm 2,4-dinitrophenol (DNP), an uncoupler of oxidative phosphorylation, coincident with an increase in glycolytic activity following embryonic genome activation. The present study examined redox-sensitive gene expression and embryo development in response to the addition of DNP post-compaction. 2,4-Dinitrophenol increased the expression of hypoxia-inducible factor 1α and 2α (HIF1α, HIF2α) mRNA. Although HIF1α protein remained undetectable in bovine blastocysts, HIF2α protein was localised within the nucleus of trophectoderm and inner cell mass (ICM) cells of blastocysts cultured in the presence or absence of DNP, with a slight increase in staining evident within the ICM in blastocysts cultured in the presence of DNP. However, the expression of GLUT1 and VEGF mRNA, genes known to be regulated by HIFs, was unaffected by the addition of DNP to the culture. Although the development of Grade 1 and 2 blastocysts was unaltered by the addition of DNP post compaction in the present study, a significant increase in the proportion of ICM cells was observed. Results indicate that 10 μm DNP improves the quality of bovine embryos, coincident with increased HIF2α protein localisation within ICM cells and increased HIFα mRNA levels. Therefore, the results demonstrate redox-regulated expression of HIF2.en
dc.language.isoenen
dc.publisherC S I R O Publishingen
dc.subjectBlastocyst; Animals; Cattle; 2,4-Dinitrophenol; Vascular Endothelial Growth Factor A; RNA, Messenger; Uncoupling Agents; Embryo Culture Techniques; Reverse Transcriptase Polymerase Chain Reaction; Gene Expression Regulation; Oxidation-Reduction; Glucose Transporter Type 1; Basic Helix-Loop-Helix Transcription Factors; Hypoxia-Inducible Factor 1, alpha Subuniten
dc.titleEffect of the oxidative phosphorylation uncoupler 2,4-dinitrophenol on hypoxia-inducible factor-regulated gene expression in bovine blastocystsen
dc.typeJournal articleen
dc.identifier.rmid0020041187en
dc.identifier.doi10.1071/RD04027en
dc.identifier.pubid56494-
pubs.library.collectionObstetrics and Gynaecology publicationsen
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidThompson, J. [0000-0003-4941-7731]en
Appears in Collections:Obstetrics and Gynaecology publications
Agriculture, Food and Wine publications

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