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Type: Journal article
Title: Effect of egg washing and correlation between eggshell characteristics and egg penetration by various Salmonella typhimurium strains
Author: Gole, V.
Chousalkar, K.
Roberts, J.
Sexton, M.
May, D.
Tan, J.
Kiermeier, A.
Citation: PLoS One, 2014; 9(3):e90987-1-e90987-12
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
Statement of
Vaibhav C. Gole, Kapil K. Chousalkar, Juliet R. Roberts, Margaret Sexton, Damian May, Jessica Tan, and Andreas Kiermeier
Abstract: Salmonella is an important foodborne pathogen, causing an estimated 11,992 cases of infection in Australia per year. Egg or egg product related salmonellosis is a major concern for the egg industry. Worldwide, S. Typhimurium is one of the most common serovars identified in Salmonella food poisoning cases. The current study investigated the ability of five S. Typhimurium strains to penetrate washed and unwashed eggs using whole egg and agar egg penetration methods. All S. Typhimurium strains were able to penetrate eggshells and survive in egg albumen (at 20°C) according to whole egg penetration results. Polymerase Chain Reaction results demonstrated that S. Typhimurium strain 2 (103 and 105 CFU/mL), and strain 5 (103 and 105 CFU/mL) egg penetration was significantly higher (p<0.05) in washed eggs when compared to unwashed eggs. Statistical analysis of the agar penetration experiment indicated that S. Typhimurium was able to penetrate washed eggs at a significantly higher rate when compared to unwashed eggs (p<0.05). When compared to unwashed eggs, washed eggs also had significantly damaged cuticles. Statistical analysis also indicated that eggshell penetration by S. Typhimurium was related to various eggshell ultrastructural features such as cap quality, alignment, erosion, confluence, Type B bodies and cuticle cover.
Keywords: Egg Shell; Animals; Salmonella typhimurium; Food Microbiology; Sanitation; Time Factors; Eggs; Microbial Viability
Rights: ©2014 Chousalkar et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020137611
DOI: 10.1371/journal.pone.0090987
Appears in Collections:Animal and Veterinary Sciences publications

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