Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/86601
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Type: Journal article
Title: Host components in hepatitis B antigen
Author: Burrell, C.
Citation: Journal of General Virology, 1975; 27(2):117-126
Publisher: Society for General Microbiology
Issue Date: 1975
ISSN: 0022-1317
1465-2099
Statement of
Responsibility: 
C. J. Burrell
Abstract: Purified [125I]-labelled 20 to 25 nm hepatitis B antigen particles were found to give low affinity immunoprecipitation reactions with antisera to several normal serum components, which were immunologically distinct from the reaction due to the classical hepatitis B antigen surface determinant. These additional antigenic determinants were acid-stable and tightly bound to the particles; they could not be released by treatment with Tween 80 or ether, but were removed by protease digestion with the preservation of particle integrity. It was not possible to distinguish whether they were due to the presence of trace amounts of partly denatured serum components, or to a weak cross-reaction with antigens present in normal serum. The implications of this finding for hepatitis B antigen and antibody detection in sensititive assays are discussed. No evidence was found for native antigenic material, present in normal serum or normal liver cells, being integral to the structure of these particles.
Keywords: Humans; Iodine Radioisotopes; Polysorbates; Bromelains; Trypsin; Lipoproteins; Serum Albumin; Immunoglobulin G; Immune Sera; Hepatitis B Antigens; Epitopes; Radioimmunoassay; Immunodiffusion; Cross Reactions
Rights: Copyright status unknown
RMID: 0030009967
DOI: 10.1099/0022-1317-27-2-117
Appears in Collections:Molecular and Biomedical Science publications

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