Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/92124
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Type: Journal article
Title: De novo transcriptome assembly and comparative analysis of differentially expressed genes in Prunus dulcis Mill. in response to freezing stress
Author: Mousavi, S.
Alisoltani, A.
Shiran, B.
Fallahi, H.
Ebrahimie, E.
Imani, A.
Houshmand, S.
Citation: PLoS One, 2014; 9(8):e104541-1-e104541-13
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
1932-6203
Statement of
Responsibility: 
Sadegh Mousavi, Arghavan Alisoltani, Behrouz Shiran, Hossein Fallahi, Esameil Ebrahimie, Ali Imani, Saadollah Houshmand
Abstract: Almond (Prunus dulcis Mill.), one of the most important nut crops, requires chilling during winter to develop fruiting buds. However, early spring chilling and late spring frost may damage the reproductive tissues leading to reduction in the rate of productivity. Despite the importance of transcriptional changes and regulation, little is known about the almond's transcriptome under the cold stress conditions. In the current research, we used RNA-seq technique to study the response of the reproductive tissues of almond (anther and ovary) to frost stress. RNA sequencing resulted in more than 20 million reads from anther and ovary tissues of almond, individually. About 40,000 contigs were assembled and annotated de novo in each tissue. Profile of gene expression in ovary showed significant alterations in 5,112 genes, whereas in anther 6,926 genes were affected by freezing stress. Around two thousands of these genes were common altered genes in both ovary and anther libraries. Gene ontology indicated the involvement of differentially expressed (DE) genes, responding to freezing stress, in metabolic and cellular processes. qRT-PCR analysis verified the expression pattern of eight genes randomly selected from the DE genes. In conclusion, the almond gene index assembled in this study and the reported DE genes can provide great insights on responses of almond and other Prunus species to abiotic stresses. The obtained results from current research would add to the limited available information on almond and Rosaceae. Besides, the findings would be very useful for comparative studies as the number of DE genes reported here is much higher than that of any previous reports in this plant.
Keywords: Prunus; Plant Proteins; Gene Expression Profiling; Sequence Analysis, RNA; Gene Expression Regulation, Plant; Genes, Plant; Freezing; Stress, Physiological; Molecular Sequence Annotation; Transcriptome
Description: Published: August 14, 2014
Rights: © 2014 Mousavi et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0030008782
DOI: 10.1371/journal.pone.0104541
Appears in Collections:Genetics publications

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