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Type: Journal article
Title: Alzheimer's disease-related peptide PS2V plays ancient, conserved roles in suppression of the unfolded protein response under hypoxia and stimulation of γ-secretase activity
Other Titles: Alzheimer's disease-related peptide PS2V plays ancient, conserved roles in suppression of the unfolded protein response under hypoxia and stimulation of gamma-secretase activity
Author: Moussavi Nik, S.
Newman, M.
Wilson, L.
Ebrahimie, E.
Wells, S.
Musgrave, I.
Verdile, G.
Martins, R.
Lardelli, M.
Citation: Human Molecular Genetics, 2015; 24(13):3662-3678
Publisher: Oxford University Press
Issue Date: 2015
ISSN: 1460-2083
Statement of
Seyyed Hani Moussavi Nik, Morgan Newman, Lachlan Wilson, Esmaeil Ebrahimie, Simon Wells, Ian Musgrave, Giuseppe Verdile, Ralph N. Martins, and Michael Lardelli
Abstract: The PRESENILIN1 and PRESENILIN2 genes encode structurally related proteases essential for γ -secretase activity. Of nearly 200 PRESENILIN mutations causing early onset, familial Alzheimer ’ s disease (FAD) only the K115Efx10 mutation of PSEN2 causes truncation of the open reading frame. If translated, the truncated product would resemble a naturally occurring isoform of PSEN2 named PS2V that is induced by hypoxia and found at elevated levels in late onset Alzheimer ’ s disease (AD) brains. The function of PS2V is largely unexplored. We show that zebra fi sh possess a PS2V-like isoform, PS1IV, produced from the fi sh ’ s PSEN1 rather than PSEN2 orthologous gene. The molecular mechanism controlling formation of PS2V/PS1IV was probably present in the ancient common ancestorof the PSEN1 and PSEN2 genes. Human PS2Vand zebra fi sh PS1IV have highly divergent structures but conserved abilities to stimulate γ -secretase activity and to suppress the unfolded protein response (UPR) under hypoxia. The putative protein truncation caused by K115Efx10 resembles PS2V in its ability to increase γ -secretase activity and suppress the UPR. This supports increased A β levels as a common link between K115Efx10 early onset AD and sporadic, late onset AD. The ability of mutant variants of PS2V to stimulate γ -secretase activity partially correlates with their ability to suppress the UPR. The cytosolic, transmembrane and luminal domains of PS2V are all critical to its γ -secretase and UPR- suppression activities. Our data support a model in which chronic hypoxia in aged brains promotes excessive Notch signalling and accumulation of A β that contribute to AD pathogenesis.
Keywords: Animals; Zebrafish; Humans; Alzheimer Disease; Peptides; Amyloid beta-Protein Precursor; Zebrafish Proteins; Membrane Proteins; Female; Male; Amyloid Precursor Protein Secretases; Presenilin-1; Presenilin-2; Unfolded Protein Response; Biological Evolution; Hypoxia
Rights: © The Author 2015.
RMID: 0030026134
DOI: 10.1093/hmg/ddv110
Grant ID:
Appears in Collections:Genetics publications

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