Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/94370
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Type: | Journal article |
Title: | Synovial fluid myeloid dendritic cells display important differences compared to monocyte-derived dendritic cells prepared in vitro |
Author: | Moghaddami, M. James, M. Whittle, S. Cleland, L. |
Citation: | Clinical & Translational Immunology, 2014; 3(8):e23-1-e23-7 |
Publisher: | Nature |
Issue Date: | 2014 |
ISSN: | 2050-0068 2050-0068 |
Statement of Responsibility: | Mahin Moghaddami, Michael James, Samuel L Whittle, and Leslie G Cleland |
Abstract: | The object of this study was to characterise synovial fluid dendritic cells (SFDCs) with regard to morphology, phenotype and responses to 1,25hydroxy-cholecalciferol (1,25D) and lipopolysaccharide (LPS), and to compare these characteristics with those of peripheral blood (PB) monocyte-derived DCs (MDDCs). SF was aspirated from knees with inflammatory effusions. PB samples were obtained contemporaneously. SFDCs were separated by flow cytometry. Morphology was determined on cytosmears. Expression of accessory molecules, cytokines and prostaglandin synthases mRNA was quantified by reverse transcription PCR. Analyses were performed on freshly prepared DCs and after incubation with 1,25D and LPS, separately and in combination. SFDCs and MDDCs displayed broadly similar morphology. Expression of accessory molecules, cytokines, cyclooxygenase-2 (COX-2) and prostaglandin E-synthase (PGES) was similar. SFDCs, but not MDDCs, expressed prostaglandin D-synthase (PGDS). PGDS was lost on incubation with SFDCs, but was induced by 1,25D in MDDCs. LPS in the presence or absence of 1,25D, induced interleukin 23 (IL23), IL1β and tumour necrosis factor-α in SFDCs and MDDCs, with SFDC showing stronger expression of these cytokines. 1,25D in combination with LPS induced PGES and enhanced LPS induction of IL6 in SFDCs and MDDCs. LPS reduced 1,25D-induced expression of PGDS in MDDCs. SFDCs and MDDCs display similar basal characteristics but differ in PGDS expression and responsiveness to LPS and 1,25D. MDDCs have limitations as a model of SFDCs which have differentiated in vivo. |
Rights: | This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/3.0/. |
DOI: | 10.1038/cti.2014.19 |
Published version: | http://www.nature.com/cti/journal/v3/n8/full/cti201419a.html |
Appears in Collections: | Aurora harvest 7 Medicine publications |
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