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https://hdl.handle.net/2440/9569
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Type: | Journal article |
Title: | The role of cytochrome c in caspase activation in Drosophila melanogaster cells |
Author: | Dorstyn, L. Read, S. Cakouros, D. Huh, J. Hay, B. Kumar, S. |
Citation: | The Journal of Cell Biology, 2002; 156(6):1089-1098 |
Publisher: | Rockefeller Univ Press |
Issue Date: | 2002 |
ISSN: | 0021-9525 1540-8140 |
Statement of Responsibility: | Loretta Dorstyn, Stuart Read, Dimitrios Cakouros, Jun R. Huh, Bruce A. Hay and Sharad Kumar |
Abstract: | The release of cytochrome c from mitochondria is necessary for the formation of the Apaf-1 apoptosome and subsequent activation of caspase-9 in mammalian cells. However, the role of cytochrome c in caspase activation in Drosophila cells is not well understood. We demonstrate here that cytochrome c remains associated with mitochondria during apoptosis of Drosophila cells and that the initiator caspase DRONC and effector caspase DRICE are activated after various death stimuli without any significant release of cytochrome c in the cytosol. Ectopic expression of the proapoptotic Bcl-2 protein, DEBCL, also fails to show any cytochrome c release from mitochondria. A significant proportion of cellular DRONC and DRICE appears to localize near mitochondria, suggesting that an apoptosome may form in the vicinity of mitochondria in the absence of cytochrome c release. In vitro, DRONC was recruited to a >700-kD complex, similar to the mammalian apoptosome in cell extracts supplemented with cytochrome c and dATP. These results suggest that caspase activation in insects follows a more primitive mechanism that may be the precursor to the caspase activation pathways in mammals. |
Keywords: | DRONC caspase activation DRICE apoptosis apoptosome |
Description: | © The Rockefeller University Press |
DOI: | 10.1083/jcb.200111107 |
Published version: | http://jcb.rupress.org/cgi/content/abstract/156/6/1089 |
Appears in Collections: | Aurora harvest 4 Medicine publications |
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