Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/97185
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dc.contributor.authorGuo, Z.en
dc.contributor.authorKang, S.en
dc.contributor.authorChen, D.en
dc.contributor.authorWu, Q.en
dc.contributor.authorWang, S.en
dc.contributor.authorXie, W.en
dc.contributor.authorZhu, X.en
dc.contributor.authorBaxter, S.en
dc.contributor.authorZhou, X.en
dc.contributor.authorJurat-Fuentes, J.en
dc.contributor.authorZhang, Y.en
dc.date.issued2015en
dc.identifier.citationPLoS Genetics, 2015; 11(4):e1005124-1-e1005124-32en
dc.identifier.issn1553-7404en
dc.identifier.issn1553-7404en
dc.identifier.urihttp://hdl.handle.net/2440/97185-
dc.description.abstractInsecticidal crystal toxins derived from the soil bacterium Bacillus thuringiensis (Bt) are widely used as biopesticide sprays or expressed in transgenic crops to control insect pests. However, large-scale use of Bt has led to field-evolved resistance in several lepidopteran pests. Resistance to Bt Cry1Ac toxin in the diamondback moth, Plutella xylostella (L.), was previously mapped to a multigenic resistance locus (BtR-1). Here, we assembled the 3.15 Mb BtR-1 locus and found high-level resistance to Cry1Ac and Bt biopesticide in four independent P. xylostella strains were all associated with differential expression of a midgut membrane-bound alkaline phosphatase (ALP) outside this locus and a suite of ATP-binding cassette transporter subfamily C (ABCC) genes inside this locus. The interplay between these resistance genes is controlled by a previously uncharacterized trans-regulatory mechanism via the mitogen-activated protein kinase (MAPK) signaling pathway. Molecular, biochemical, and functional analyses have established ALP as a functional Cry1Ac receptor. Phenotypic association experiments revealed that the recessive Cry1Ac resistance was tightly linked to down-regulation of ALP, ABCC2 and ABCC3, whereas it was not linked to up-regulation of ABCC1. Silencing of ABCC2 and ABCC3 in susceptible larvae reduced their susceptibility to Cry1Ac but did not affect the expression of ALP, whereas suppression of MAP4K4, a constitutively transcriptionally-activated MAPK upstream gene within the BtR-1 locus, led to a transient recovery of gene expression thereby restoring the susceptibility in resistant larvae. These results highlight a crucial role for ALP and ABCC genes in field-evolved resistance to Cry1Ac and reveal a novel trans-regulatory signaling mechanism responsible for modulating the expression of these pivotal genes in P. xylostella.en
dc.description.statementofresponsibilityZhaojiang Guo, Shi Kang, Defeng Chen, Qingjun Wu, Shaoli Wang, Wen Xie, Xun Zhu, Simon W. Baxter, Xuguo Zhou, Juan Luis Jurat-Fuentes, Youjun Zhangen
dc.language.isoenen
dc.publisherPublic Library of Scienceen
dc.rights© 2015 Guo et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are crediteden
dc.subjectIntestinal Mucosa; Animals; Moths; Bacillus; Alkaline Phosphatase; Bacterial Proteins; ATP-Binding Cassette Transporters; Insect Proteins; Endotoxins; MAP Kinase Signaling System; Protein Binding; Insecticide Resistance; Hemolysin Proteins; Biological Control Agentsen
dc.titleMAPK signaling pathway alters expression of midgut ALP and ABCC genes and causes resistance to Bacillus thuringiensis Cry1Ac toxin in diamondback mothen
dc.typeJournal articleen
dc.identifier.rmid0030029915en
dc.identifier.doi10.1371/journal.pgen.1005124en
dc.identifier.pubid184217-
pubs.library.collectionGenetics publicationsen
pubs.library.teamDS01en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidBaxter, S. [0000-0001-5773-6578]en
Appears in Collections:Genetics publications

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