Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/99155
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Type: | Journal article |
Title: | A rapid and quantitative flow cytometry method for the analysis of membrane disruptive antimicrobial activity |
Author: | O'Brien-Simpson, N. Pantarat, N. Attard, T. Walsh, K. Reynolds, E. |
Citation: | PLoS One, 2016; 11(3):e0151694-1-e0151694-15 |
Publisher: | Public Library of Science |
Issue Date: | 2016 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Castanho, M. |
Statement of Responsibility: | Neil M. O, Brien-Simpson, Namfon Pantarat, Troy J. Attard, Katrina A. Walsh, Eric C. Reynolds |
Abstract: | We describe a microbial flow cytometry method that quantifies within 3 hours antimicrobial peptide (AMP) activity, termed Minimum Membrane Disruptive Concentration (MDC). Increasing peptide concentration positively correlates with the extent of bacterial membrane disruption and the calculated MDC is equivalent to its MBC. The activity of AMPs representing three different membranolytic modes of action could be determined for a range of Gram positive and negative bacteria, including the ESKAPE pathogens, E. coli and MRSA. By using the MDC50 concentration of the parent AMP, the method provides high-throughput, quantitative screening of AMP analogues. A unique feature of the MDC assay is that it directly measures peptide/bacteria interactions and lysed cell numbers rather than bacteria survival as with MIC and MBC assays. With the threat of multi-drug resistant bacteria, this high-throughput MDC assay has the potential to aid in the development of novel antimicrobials that target bacteria with improved efficacy. |
Keywords: | Cell Membrane Gram-Negative Bacteria Escherichia coli Gram-Positive Bacteria Antimicrobial Cationic Peptides Anti-Bacterial Agents Flow Cytometry Microbial Sensitivity Tests Methicillin-Resistant Staphylococcus aureus High-Throughput Screening Assays |
Rights: | © 2016 O’Brien-Simpson et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0151694 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/1008106 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0151694 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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hdl_99155.pdf | Published version | 1.94 MB | Adobe PDF | View/Open |
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