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https://hdl.handle.net/2440/99395
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Type: | Journal article |
Title: | Fluorescent IGF-II analogues for FRET-based investigations into the binding of IGF-II to the IGF-1R |
Author: | Cottam Jones, J. Harris, P. Scanlon, D. Forbes, B. Brimble, M. Abell, A. |
Citation: | Organic and Biomolecular Chemistry, 2016; 14(9):2698-2705 |
Publisher: | Royal Society of Chemistry |
Issue Date: | 2016 |
ISSN: | 1477-0520 1477-0539 |
Statement of Responsibility: | J. M. Cottam Jones, P. W. R. Harris, D. B. Scanlon, B. E. Forbes, M. A. Brimble and A. D. Abell |
Abstract: | The interaction of IGF-II with the insulin receptor (IR) and type 1 insulin-like growth factor receptor (IGF-1R) has recently been identified as potential therapeutic target for the treatment of cancer. Understanding the interactions of IGF-II with these receptors is required for the development of potential anticancer therapeutics. This work describes an efficient convergent synthesis of native IGF-II and two non-native IGF-II analogues with coumarin fluorescent probes incorporated at residues 19 and 28. These fluorescent analogues bind with nanomolar affinities to the IGF-1R and are suitable for use in fluorescence resonance energy transfer (FRET) studies. From these studies the F19Cou IGF-II and F28Cou IGF-II proteins were identified as good probes for investigating the binding interactions of IGF-II with the IGF-1R and its other high affinity binding partners. |
Keywords: | Receptor, IGF Type 1 Insulin-Like Growth Factor II Fluorescence Resonance Energy Transfer Binding Sites Molecular Structure Fluorescence |
Rights: | Copyright status unknown |
DOI: | 10.1039/c5ob02110c |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1039/c5ob02110c |
Appears in Collections: | Aurora harvest 3 Chemistry publications |
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