Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/102966
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Type: | Journal article |
Title: | Anti-inflammatory deficiencies in neutrophilic asthma: reduced galectin-3 and IL-1RA/IL-1β |
Other Titles: | Anti-inflammatory deficiencies in neutrophilic asthma: reduced galectin-3 and IL-1RA/IL-1beta |
Author: | Gao, P. Gibson, P. Baines, K. Yang, I. Upham, J. Reynolds, P. Hodge, S. James, A. Jenkins, C. Peters, M. Zhang, J. Simpson, J. |
Citation: | Respiratory Research, 2015; 16(1):5-1-5-10 |
Publisher: | BioMed Central |
Issue Date: | 2015 |
ISSN: | 1465-9921 1465-993X |
Statement of Responsibility: | Peng Gao, Peter G Gibson, Katherine J Baines, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Jie Zhang, and Jodie L Simpson |
Abstract: | Background: Galectin-3 (gal-3), a member of the β-galactoside-binding animal lectins, is involved in the recruitment, activation and removal of neutrophils. Neutrophilic asthma is characterized by a persistent elevation of airway neutrophils and impaired efferocytosis. We hypothesized that sputum gal-3 would be reduced in neutrophilic asthma and the expression of gal-3 would be associated with other markers of neutrophilic inflammation. Methods: Adults with asthma (n = 80) underwent a sputum induction following clinical assessment and blood collection. Sputum was dispersed for a differential cell count and ELISA assessment of gal-3, gal-3 binding protein (BP), interleukin (IL)-1β, IL-1 receptor antagonist (RA), IL-8 and IL-6. Gal-3 and gal-3BP immunoreactivity were assessed in mixed sputum cells. Results: Sputum gal-3 (median, (q1,q3)) was significantly reduced in neutrophilic asthma (183 ng/mL (91,287)) compared with eosinophilic (293 ng/mL (188,471), p = 0.021) and paucigranulocytic asthma (399 ng/mL (213,514), p = 0.004). The gal-3/gal-3BP ratio and IL-1RA/IL-1β ratio were significantly reduced, while gal-3BP and IL-1β were significantly elevated in neutrophilic asthma compared with eosinophilic and paucigranulocytic asthma. Conclusion: Patients with neutrophilic asthma have impairment in anti-inflammatory ratio of gal-3/gal-3BP and IL-1RA/IL-1β which provides a further framework for exploration into pathologic mechanisms of asthma phenotypes. |
Keywords: | Asthma; Galectin-3; Induced sputum; Neutrophil; Macrophage; IL-1β; lectin |
Rights: | © 2015 Gao et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
DOI: | 10.1186/s12931-014-0163-5 |
Published version: | http://dx.doi.org/10.1186/s12931-014-0163-5 |
Appears in Collections: | Aurora harvest 3 Medicine publications |
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hdl_102966.pdf | Published version | 2.66 MB | Adobe PDF | View/Open |
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