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https://hdl.handle.net/2440/17456
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Type: | Journal article |
Title: | Stromal-derived factor-1 promotes the growth, survival, and development of human bone marrow stromal stem cells |
Author: | Kortesidis, A. Zannettino, A. Isenmann, S. Shi, S. Lapidot, T. Gronthos, S. |
Citation: | Blood, 2005; 105(10):3793-3801 |
Publisher: | Amer Soc Hematology |
Issue Date: | 2005 |
ISSN: | 0006-4971 1528-0020 |
Abstract: | The maintenance of bone marrow stromal stem cells (BMSSCs) is tightly controlled by the local microenvironment and by autocrine regulatory factors secreted by BMSSCs. To identify such factors, a cDNA subtraction library was generated from purified BMSSCs, based on their high expression of the STRO-1 antigen. Stromal-derived factor-1 (SDF-1) was one differentially expressed gene highly expressed by purified BMSSCs prior to culture. In vitro, immature preosteogenic cells expressed greater levels of SDF-1 when compared with mature cell types representative of osteoblasts and osteocytes/bone lining cells. Furthermore, SDF-1 expression was rapidly down-regulated when BMSSCs were cultured under osteoinductive conditions. BMSSCs were also shown to express functional cell surface SDF-1 receptors (CXCR4). Transduced BMSSC lines, secreting high SDF-1 levels, displayed an enhanced ability to form ectopic bone in vivo, in comparison with control BMSSC lines. Moreover, high SDF-1-expressing BMSSCs displayed an increased capacity for cellular growth and protection against interleukin-4-induced apoptosis. Similarly, fibroblast colony-forming units (CFU-Fs) also displayed increased growth and resistance to alpha-interferon-2a-induced apoptosis, in synergy with platelet-derived growth factor BB (PDGF-BB) and SDF-1 in vitro. These studies indicate that the chemokine, SDF-1, may play a role in the maintenance, survival, and osteogenic capacity of immature BMSSC populations. |
Keywords: | Bone and Bones Bone Marrow Cells Stromal Cells Humans Receptors, CXCR4 Chemokines, CXC Cell Differentiation Cell Proliferation Cell Survival Osteogenesis Adult Female Male Chemokine CXCL12 |
DOI: | 10.1182/blood-2004-11-4349 |
Published version: | http://dx.doi.org/10.1182/blood-2004-11-4349 |
Appears in Collections: | Aurora harvest 6 Medicine publications |
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