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https://hdl.handle.net/2440/23893
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Type: | Journal article |
Title: | Single nucleotide polymorphic marker enabling rapid and early screening for the homoeolocus of β-amylase-R1: a gene linked to copper efficiency on 5RL |
Other Titles: | Single nucleotide polymorphic marker enabling rapid and early screening for the homoeolocus of beta-amylase-R1: a gene linked to copper efficiency on 5RL |
Author: | Leach, R. Dundas, I. |
Citation: | Theoretical and Applied Genetics: international journal of plant breeding research, 2006; 113(2):301-307 |
Publisher: | Springer |
Issue Date: | 2006 |
ISSN: | 0040-5752 1432-2242 |
Abstract: | This study describes the development of a PCR marker to detect the β-amylase-R1 gene of rye. It provides an easy and rapid means for the identification of plants containing the β-amylase-R1. Because rye chromosome segments do not normally recombine with wheat chromosomes, this marker provides a means for tracking all linked genes on that alien 5RL chromosome segment. Reaction conditions were optimised for an annealing temperature of 60°C for a high stringency. The reaction was also optimised for low reaction volumes reducing the cost of the reagents required for the reaction. This PCR test can be used in breeding or mapping programs for the rapid screening of progeny containing translocations of 5RL and hence select for the copper efficiency trait of rye. |
Keywords: | Triticum Copper beta-Amylase DNA, Plant DNA Primers Genetic Markers Polymerase Chain Reaction Base Sequence Sequence Homology, Nucleic Acid Polymorphism, Single Nucleotide Molecular Sequence Data |
Description: | The original publication can be found at www.springerlink.com |
DOI: | 10.1007/s00122-006-0296-0 |
Published version: | http://dx.doi.org/10.1007/s00122-006-0296-0 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 2 |
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