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https://hdl.handle.net/2440/28030
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Type: | Journal article |
Title: | Stimulation of human neutrophils by chemotactic factors is associated with the activation of phosphatidylinositol 3-kinase g |
Author: | Naccache, P. Levasseur, S. Lachance, G. Chakravarti, S. Bourgoin, S. McColl, S. |
Citation: | Journal of Biological Chemistry, 2000; 275(31):23636-23641 |
Publisher: | Amer Soc Biochemistry Molecular Biology Inc |
Issue Date: | 2000 |
ISSN: | 0021-9258 1083-351X |
Abstract: | The activation of human polymorphonuclear neutrophil leukocytes (neutrophils) is associated with an increased synthesis of the highly phosphorylated phosphatidylinositol 3,4,5-trisphosphate (PtdIns(3,4,5)P(3)). The aims of the present investigation were to determine whether the newly described, G protein-dependent phosphatidylinositol 3-kinase (PI3K), p110gamma, was involved in the responses to chemotactic factors interacting with G protein-coupled receptors. The presence of p110gamma in neutrophils was first established both at the protein and the mRNA level. Stimulation of the cells with fMet-Leu-Phe or interleukin-8 increased the PI3K activity in p110gamma, but not p85, immunoprecipitates. The time course of this effect (threshold within less than 5 s, maximal activation at 10-15 s) was consistent with that of the generation of PtdIns(3,4,5)P(3). Wortmannin, a PI3K inhibitor, abrogated the effects of fMet-Leu-Phe, which were also significantly inhibited by pertussis toxin. Finally, fMet-Leu-Phe also induced a significant translocation of p110gamma to a particulate fraction derived from these cells. These data indicate that p110gamma represent the major PI3K activated by fMet-Leu-Phe and interleukin-8 at very early time points following the stimulation of human neutrophils. |
Keywords: | Humans Androstadienes Isoenzymes Pertussis Toxin N-Formylmethionine Leucyl-Phenylalanine RNA, Messenger Interleukin-8 Chemotactic Factors Virulence Factors, Bordetella Cell Compartmentation Neutrophil Activation Biological Transport Adult Phosphatidylinositol 3-Kinases Wortmannin Phosphoinositide-3 Kinase Inhibitors |
DOI: | 10.1074/jbc.M001780200 |
Published version: | http://dx.doi.org/10.1074/jbc.m001780200 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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