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https://hdl.handle.net/2440/28088
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Type: | Journal article |
Title: | Production of chemokines in vivo in response to microbial stimulation |
Author: | Coates, N. McColl, S. |
Citation: | Journal of Immunology, 2001; 166(8):5176-5182 |
Publisher: | Amer Assoc Immunologists |
Issue Date: | 2001 |
ISSN: | 0022-1767 1550-6606 |
Statement of Responsibility: | Nicholas J. Coates and Shaun R. McColl |
Abstract: | Members of the chemokine gene superfamily are known to play a central role in leukocyte extravasation; however, their involvement in acute inflammation in response to micro-organisms has not yet been well studied. We have therefore investigated the role of murine macrophage-inflammatory protein (muMIP) 1 and muMIP-2 in the inflammatory response mounted against the bacteria Salmonella enteritidis and the Sacchromyces cerevisiae cell wall component, zymosan. Leukocyte extravasation was monitored in murine s.c. air pouches. Both agonists induced accumulation of leukocytes in a dose- and time-dependent manner, with the response peaking after 4 h and declining thereafter. The inflammatory exudate comprised mainly neutrophils; however, an increase in eosinophil accumulation was also observed in response to zymosan. The production of both muMIP-1 and muMIP-2 increased with time in response to both the agonists, although production was more sustained in response to the bacteria. Prior treatment of mice with neutralizing Abs against muMIP-1 or muMIP-2, either alone or in combination, failed to attenuate the accumulation of leukocytes in response to the agonists. In contrast, the anti-muMIP-2 Abs significantly inhibited leukocyte recruitment in response to S. enteritidis in complement-deficient mice. Taken together, these data show that while muMIP-1 and muMIP-2 are produced in response to phagocytosis of micro-organisms in s.c. tissue, under these circumstances components of the complement pathway appear to play a dominant role in the recruitment of neutrophils. |
Keywords: | Phagocytes Exudates and Transudates Animals Mice, Inbred BALB C Mice Mice, Mutant Strains Salmonella enteritidis Saccharomyces cerevisiae Sodium Chloride Phosphates Zymosan Chemokines Macrophage Inflammatory Proteins Immune Sera Buffers Diffusion Chambers, Culture Injections, Intraperitoneal Injections, Subcutaneous Cell Movement Phagocytosis Down-Regulation Complement C5 Female Male Chemokine CCL4 Chemokine CXCL2 |
Description: | Copyright © 2001 by The American Association of Immunologists |
DOI: | 10.4049/jimmunol.166.8.5176 |
Published version: | http://www.jimmunol.org/cgi/content/abstract/166/8/5176 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
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