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https://hdl.handle.net/2440/2970
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Type: | Journal article |
Title: | A RhoGEF and Rho family GTPase-activating protein complex links the contractile ring to cortical microtubules at the onset of cytokinesis |
Author: | Somers, W. Saint, R. |
Citation: | Developmental Cell, 2003; 4(1):29-39 |
Publisher: | Cell Press |
Issue Date: | 2003 |
ISSN: | 1534-5807 1878-1551 |
Organisation: | Centre for the Molecular Genetics of Development |
Abstract: | The mechanism that positions the cytokinetic contractile ring is unknown, but derives from the spindle midzone. We show that an interaction between the Rho GTP exchange factor, Pebble, and the Rho family GTPase-activating protein, RacGAP50C, connects the contractile ring to cortical microtubules at the site of furrowing in D. melanogaster cells. Pebble regulates actomyosin organization, while RacGAP50C and its binding partner, the Pavarotti kinesin-like protein, regulate microtubule bundling. All three factors are required for cytokinesis. As furrowing begins, these proteins colocalize to a cortical equatorial ring. We propose that RacGAP50C-Pavarotti complexes travel on cortical microtubules to the cell equator, where they associate with the Pebble RhoGEF to position contractile ring formation and coordinate F-actin and microtubule remodeling during cytokinesis. |
Keywords: | Microtubules Animals Drosophila melanogaster Macromolecular Substances GTPase-Activating Proteins Guanine Nucleotide Exchange Factors Microtubule-Associated Proteins Drosophila Proteins RNA, Messenger Two-Hybrid System Techniques Cell Division Protein Binding Rho Guanine Nucleotide Exchange Factors Spindle Apparatus Wings, Animal |
DOI: | 10.1016/S1534-5807(02)00402-1 |
Published version: | http://dx.doi.org/10.1016/s1534-5807(02)00402-1 |
Appears in Collections: | Aurora harvest 6 Centre for the Molecular Genetics of Development publications Molecular and Biomedical Science publications |
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