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https://hdl.handle.net/2440/44455
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Type: | Journal article |
Title: | Reduced cortical inhibition in a mouse model of familial childhood absence epilepsy |
Author: | Tan, H. Reid, C. Single, F. Davies, P. Chiu, C. Murphy, S. Clarke, A. Dibbens, L. Krestel, H. Mulley, J. Jones, M. Seeburg, P. Sakmann, B. Berkovic, S. Sprengel, R. Petrou, S. |
Citation: | Proceedings of the National Academy of Sciences of USA, 2007; 104(44):17536-17541 |
Publisher: | Natl Acad Sciences |
Issue Date: | 2007 |
ISSN: | 0027-8424 1091-6490 |
Statement of Responsibility: | Heneu O. Tan, Christopher A. Reid, Frank N. Single, Philip J. Davies, Cindy Chiu, Susan Murphy, Alison L. Clarke, Leanne Dibbens, Heinz Krestel, John C. Mulley, Mathew V. Jones, Peter H. Seeburg, Bert Sakmann, Samuel F. Berkovic, Rolf Sprengel and Steven Petrou |
Abstract: | Mutations in the GABAA receptor 2 subunit are associated with childhood absence epilepsy and febrile seizures. To understand better the molecular basis of absence epilepsy in man, we developed a mouse model harboring a 2 subunit point mutation (R43Q) found in a large Australian family. Mice heterozygous for the mutation demonstrated behavioral arrest associated with 6-to 7-Hz spike-and-wave discharges, which are blocked by ethosuximide, a first-line treatment for absence epilepsy in man. Seizures in the mouse showed an abrupt onset at around age 20 days corresponding to the childhood nature of this disease. Reduced cell surface expression of 2(R43Q) was seen in heterozygous mice in the absence of any change in 1 subunit surface expression, ruling out a dominant-negative effect. GABAA-mediated synaptic currents recorded from cortical pyramidal neurons revealed a small but significant reduction that was not seen in the reticular or ventrobasal thalamic nuclei. We hypothesize that a subtle reduction in cortical inhibition underlies childhood absence epilepsy seen in humans harboring the R43Q mutation. |
Keywords: | Animals Mice Epilepsy, Absence Disease Models, Animal Genetic Predisposition to Disease Ethosuximide Receptors, GABA-A Protein Subunits Phenotype |
Rights: | Copyright © 2007 by The National Academy of Sciences of the USA |
DOI: | 10.1073/pnas.0708440104 |
Published version: | http://www.pnas.org/cgi/reprint/0708440104v1.pdf |
Appears in Collections: | Aurora harvest 6 Molecular and Biomedical Science publications |
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