Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/44832
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Sphingosine kinase 1 is a critical component of the copper-dependent FGF1 export pathway |
Author: | Soldi, R. Mandinova, A. Venkataraman, K. Hla, T. Vadas, M. Pitson, S. Duarte, M. Graziani, I. Kolev, V. Kacer, D. Kirov, A. Maciag, T. Prudovsky, I. |
Citation: | Experimental Cell Research, 2007; 313(15):3308-3318 |
Publisher: | Academic Press Inc Elsevier Science |
Issue Date: | 2007 |
ISSN: | 0014-4827 1090-2422 |
Statement of Responsibility: | Raffaella Soldi, Anna Mandinova, Krishnan Venkataraman, Timoty Hla, Mathew Vadas, Stuart Pitson, Maria Duarte, Irene Graziani, Vihren Kolev, Doreen Kacer, Aleksandr Kirov, Thomas Maciag and Igor Prudovsky |
Abstract: | Sphingosine kinase 1 catalyzes the formation of sphingosine-1-phosphate, a lipid mediator involved in the regulation of angiogenesis. Sphingosine kinase 1 is constitutively released from cells, even though it lacks a classical signal peptide sequence. Because copper-dependent non-classical stress-induced release of FGF1 also regulates angiogenesis, we questioned whether sphingosine kinase 1 is involved in the FGF1 release pathway. We report that (i) the coexpression of sphingosine kinase 1 with FGF1 inhibited the release of sphingosine kinase 1 at 37 degrees C; (ii) sphingosine kinase 1 was released at 42 degrees C in complex with FGF1; (iii) sphingosine kinase 1 null cells failed to release FGF1 at stress; (iv) sphingosine kinase 1 is a high affinity copper-binding protein which formed a complex with FGF1 in a cell-free system, and (v) sphingosine kinase 1 over expression rescued the release of FGF1 from inhibition by the copper chelator, tetrathiomolybdate. We propose that sphingosine kinase 1 is a component of the copper-dependent FGF1 release pathway. |
Keywords: | Cells, Cultured NIH 3T3 Cells Fibroblasts Animals Mice, Knockout Mice Copper Molybdenum Phosphotransferases (Alcohol Group Acceptor) Fibroblast Growth Factor 1 Chelating Agents Cloning, Molecular Temperature Protein Transport |
Description: | Copyright © 2007 Elsevier Inc. All rights reserved. |
DOI: | 10.1016/j.yexcr.2007.05.031 |
Description (link): | http://www.elsevier.com/wps/find/journaldescription.cws_home/622826/description#description |
Published version: | http://dx.doi.org/10.1016/j.yexcr.2007.05.031 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.