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https://hdl.handle.net/2440/50661
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Type: | Journal article |
Title: | Novel allelic variants encoded at the Glu-D3 locus in bread wheat |
Author: | Appelbee, M. Mekuria, G. Nagasandra, V. Bonneau, J. Eagles, H. Eastwood, R. Mather, D. |
Citation: | Journal of Cereal Science, 2009; 49(2):254-261 |
Publisher: | Academic Press Ltd Elsevier Science Ltd |
Issue Date: | 2009 |
ISSN: | 0733-5210 1095-9963 |
Statement of Responsibility: | M.-J. Appelbee, G.T. Mekuria, V. Nagasandra, J.P. Bonneau, H.A. Eagles, R.F. Eastwood and D.E. Mather |
Abstract: | Low-molecular weight glutenin subunits (LWM-GS) are important components of wheat (Triticum aestivum L.) gluten, with important effects on end-use quality. The LMW-GS are encoded at Glu-3 loci (Glu-A3, Glu-B3 and Glu-D3, on the short arms of chromosomes 1A, 1B and 1D), each of which exhibits extensive allelic variation. Each locus encodes numerous LMW-GS, some of which have similar electrophoretic mobilities, making it difficult to distinguish among Glu-3 loci. Alleles of the Glu-D3 locus of bread wheat are considered the most problematic to assign. To date, six Glu-D3 alleles, designated a, b, c, d, e and f, have been reported. We report five previously undescribed alleles (g, h, i, j and k), and describe a method for characterizing them using a combination of SDS-PAGE and multiplexed PCR-based DNA markers. This method could be used for accurate identification of Glu-D3 alleles, permitting the estimation of the effects of these alleles on end-use quality and the selection of desirable alleles and allelic combinations in wheat breeding. |
Keywords: | Glu-3 Glu-D3 Low-molecular weight glutenin Protein Wheat |
Rights: | Crown copyright © 2008 Published by Elsevier Ltd. |
DOI: | 10.1016/j.jcs.2008.10.011 |
Published version: | http://dx.doi.org/10.1016/j.jcs.2008.10.011 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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