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https://hdl.handle.net/2440/56836
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dc.contributor.author | Preuss, C. | - |
dc.contributor.author | Huang, C. | - |
dc.contributor.author | Gilliham, M. | - |
dc.contributor.author | Tyerman, S. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Plant Physiology, 2010; 152(3):1431-1441 | - |
dc.identifier.issn | 0032-0889 | - |
dc.identifier.issn | 1532-2548 | - |
dc.identifier.uri | http://hdl.handle.net/2440/56836 | - |
dc.description.abstract | Remobilization of inorganic phosphate (Pi) within a plant is critical for sustaining growth and seed production under external Pi fluctuation. The barley (Hordeum vulgare) transporter HvPHT1;6 has been implicated in Pi remobilization. In this report, we expressed HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6. HvPHT1;6 increased efflux of Pi near oocyte resting membrane potentials, dependent on external Pi concentration. Time-dependent inward currents were observed when membrane potentials were more negative than –160 mV, which was consistent with nH+:HPO42– (n > 2) cotransport, based on simultaneous radiotracer and oocyte voltage clamping, dependent upon Pi concentration gradient and pH. Time- and voltage-dependent inward currents through HvPHT1;6 were also observed for SO42–and to a lesser degree for NO3–Cl–but not for malate. Inward and outward currents showed linear dependence on the concentration of external HPO42–similar to low-affinity Pi transport in plant studies. The electrophysiological properties of HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of Pi in barley plants. | - |
dc.description.statementofresponsibility | Christian P. Preuss, Chun Y. Huang, Matthew Gilliham, and Stephen D. Tyerman | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Plant Physiologists | - |
dc.rights | © 2010 American Society of Plant Biologists | - |
dc.source.uri | http://dx.doi.org/10.1104/pp.109.152009 | - |
dc.subject | Oocytes | - |
dc.subject | Animals | - |
dc.subject | Xenopus | - |
dc.subject | Hordeum | - |
dc.subject | Phosphates | - |
dc.subject | Phosphate Transport Proteins | - |
dc.subject | Plant Proteins | - |
dc.subject | DNA, Plant | - |
dc.subject | Cloning, Molecular | - |
dc.subject | Membrane Potentials | - |
dc.title | Channel-like characteristics of the low affinity barley phosphate transporter Pht1;6 when expressed in Xenopus Oocytes1,[W],[OA] | - |
dc.type | Journal article | - |
dc.contributor.organisation | Australian Centre for Plant Functional Genomics (ACPFG) | - |
dc.identifier.doi | 10.1104/pp.109.152009 | - |
dc.relation.grant | ARC | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Gilliham, M. [0000-0003-0666-3078] | - |
dc.identifier.orcid | Tyerman, S. [0000-0003-2455-1643] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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