Adelaide Research & Scholarship
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https://hdl.handle.net/2440/59512
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| Type: | Journal article |
| Title: | aac(6')-Ib-cr genotyping by simultaneous high-resolution melting analyses of an unlabeled probe and full-length amplicon |
| Author: | Bell, J. Turnidge, J. Andersson, P. |
| Citation: | Antimicrobial Agents and Chemotherapy, 2010; 54(3):1378-1380 |
| Publisher: | Amer Soc Microbiology |
| Issue Date: | 2010 |
| ISSN: | 0066-4804 1098-6596 |
| Statement of Responsibility: | Jan M. Bell, John D. Turnidge and Patiyan Andersson |
| Abstract: | We have developed a time- and cost-efficient one-step closed-tube assay for genotyping of aac(6')-Ib-cr that is capable of distinguishing between the two genetic aac(6')-Ib-cr variants. Our genotyping assay uses the combined information of simultaneously acquired high-resolution melting data from an unlabeled probe and the full-length amplicon. |
| Keywords: | Humans Enterobacteriaceae Acetyltransferases Oligonucleotide Probes Microbial Sensitivity Tests Polymerase Chain Reaction Genotype Polymorphism, Restriction Fragment Length Transition Temperature Genetic Variation |
| Rights: | Copyright © 2010, American Society for Microbiology. All Rights Reserved. |
| DOI: | 10.1128/AAC.01476-09 |
| Published version: | http://dx.doi.org/10.1128/aac.01476-09 |
| Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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