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https://hdl.handle.net/2440/59941
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dc.contributor.author | Brotherton, P. | - |
dc.contributor.author | Sanchez, J. | - |
dc.contributor.author | Cooper, A. | - |
dc.contributor.author | Endicott, P. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Nucleic Acids Research, 2010; 38(2):E7-1-E7-12 | - |
dc.identifier.issn | 0305-1048 | - |
dc.identifier.issn | 1362-4962 | - |
dc.identifier.uri | http://hdl.handle.net/2440/59941 | - |
dc.description.abstract | The analysis of targeted genetic loci from ancient, forensic and clinical samples is usually built upon polymerase chain reaction (PCR)-generated sequence data. However, many studies have shown that PCR amplification from poor-quality DNA templates can create sequence artefacts at significant levels. With hominin (human and other hominid) samples, the pervasive presence of highly PCR-amplifiable human DNA contaminants in the vast majority of samples can lead to the creation of recombinant hybrids and other non-authentic artefacts. The resulting PCR-generated sequences can then be difficult, if not impossible, to authenticate. In contrast, single primer extension (SPEX)-based approaches can genotype single nucleotide polymorphisms from ancient fragments of DNA as accurately as modern DNA. A single SPEX-type assay can amplify just one of the duplex DNA strands at target loci and generate a multi-fold depth-of-coverage, with non-authentic recombinant hybrids reduced to undetectable levels. Crucially, SPEX-type approaches can preferentially access genetic information from damaged and degraded endogenous ancient DNA templates over modern human DNA contaminants. The development of SPEX-type assays offers the potential for highly accurate, quantitative genotyping from ancient hominin samples. | - |
dc.description.statementofresponsibility | Paul Brotherton, Juan J. Sanchez, Alan Cooper and Phillip Endicott | - |
dc.language.iso | en | - |
dc.publisher | Oxford Univ Press | - |
dc.rights | © The Author(s) 2009. Published by Oxford University Press. | - |
dc.source.uri | http://dx.doi.org/10.1093/nar/gkp897 | - |
dc.subject | Animals | - |
dc.subject | Hominidae | - |
dc.subject | Humans | - |
dc.subject | Reproducibility of Results | - |
dc.subject | Nucleic Acid Amplification Techniques | - |
dc.subject | Polymerase Chain Reaction | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Base Sequence | - |
dc.subject | Genotype | - |
dc.subject | Polymorphism, Single Nucleotide | - |
dc.subject | Museums | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Preferential access to genetic information from endogenous hominin ancient DNA and accurate quantitative SNP-typing via SPEX | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1093/nar/gkp897 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Cooper, A. [0000-0002-7738-7851] | - |
Appears in Collections: | Aurora harvest 5 Australian Centre for Ancient DNA publications Earth and Environmental Sciences publications Environment Institute Leaders publications |
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