Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/60622
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Spatiotemporal regulation of multiple overlapping sense and novel natural antisense transcripts at the Nrgn and Camk2n1 gene loci during mouse cerebral corticogenesis |
Author: | Ling, K. Hewitt, C. Beissbarth, T. Hyde, L. Cheah, P. Smyth, G. Tan, S. Hahn, C. Thomas, T. Thomas, P. Scott, H. |
Citation: | Cerebral Cortex, 2011; 21(3):683-697 |
Publisher: | Oxford Univ Press Inc |
Issue Date: | 2011 |
ISSN: | 1047-3211 1460-2199 |
Statement of Responsibility: | King-Hwa Ling, Chelsee A. Hewitt, Tim Beissbarth, Lavinia Hyde, Pike-See Cheah, Gordon K. Smyth, Seong-Seng Tan, Christopher N. Hahn, Tim Thomas, Paul Q. Thomas and Hamish S. Scott |
Abstract: | Nrgn and Camk2n1 are highly expressed in the brain and play an important role in synaptic long-term potentiation via regulation of Ca2+/calmodulin-dependent protein kinase II. We have shown that the gene loci for these 2 proteins are actively transcribed in the adult cerebral cortex and feature multiple overlapping transcripts in both the sense and antisense orientations with alternative polyadenylation. These transcripts were upregulated in the adult compared with embryonic and P1.5 mouse cerebral cortices, and transcripts with different 3′ untranslated region lengths showed differing expression profiles. In situ hybridization (ISH) analysis revealed spatiotemporal regulation of the Nrgn and Camk2n1 sense and natural antisense transcripts (NATs) throughout cerebral corticogenesis. In addition, we also demonstrated that the expression of these transcripts was organ-specific. Both Nrgn and Camk2n1 sense and NATs were also upregulated in differentiating P19 teratocarcinoma cells. RNA fluorescent ISH analysis confirmed the capability of these NATs to form double-stranded RNA aggregates with the sense transcripts in the cytoplasm of cells obtained from the brain. We propose that the differential regulation of multiple sense and novel overlapping NATs at the Nrgn and Camk2n1 loci will increase the diversity of posttranscriptional regulation, resulting in cell- and time-specific regulation of their gene products during cerebral corticogenesis and function. |
Keywords: | CaMKII P19 teratocarcinoma cells serial analysis of gene expression and fluorescent in situ hybridization |
Description: | First published online: August 6, 2010 |
Rights: | © The Author 2010. Published by Oxford University Press. All rights reserved. |
DOI: | 10.1093/cercor/bhq141 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/171601 http://purl.org/au-research/grants/nhmrc/461204 http://purl.org/au-research/grants/nhmrc/219176 http://purl.org/au-research/grants/nhmrc/257501 http://purl.org/au-research/grants/nhmrc/215201 http://purl.org/au-research/grants/nhmrc/257529 |
Published version: | http://cercor.oxfordjournals.org/content/early/2010/08/06/cercor.bhq141.abstract |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.