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Type: Journal article
Title: Boron toxicity tolerance in barley through reduced expression of the multifunctional aquaporin HvNIP2;1
Author: Schnurbusch, T.
Hayes, J.
Hrmova, M.
Baumann, U.
Ramesh, S.
Tyerman, S.
Langridge, P.
Sutton, T.
Citation: Plant Physiology, 2010; 153(4):1706-1715
Publisher: Amer Soc Plant Physiologists
Issue Date: 2010
ISSN: 0032-0889
Statement of
Thorsten Schnurbusch, Julie Hayes, Maria Hrmova, Ute Baumann, Sunita A. Ramesh, Stephen D. Tyerman, Peter Langridge and Tim Sutton
Abstract: Boron (B) toxicity is a significant limitation to cereal crop production in a number of regions worldwide. Here we describe the cloning of a gene from barley, underlying the chromosome 6H B toxicity tolerance QTL. It is the second B toxicity tolerance gene identified in barley. Previously, we identified the gene HvBot1 which functions as an efflux transporter in B toxicity tolerant barley to move B out of the plant. The gene identified in this work encodes HvNIP2;1, an aquaporin from the NIP subfamily which was recently described as a silicon influx transporter in barley and rice. Here we show that a rice mutant for this gene also shows reduced B accumulation in leaf blades compared to wild type and that the mutant protein alters growth of yeast under high B. HvNIP2;1 facilitates significant transport of B when expressed in Xenopus oocytes compared to controls and to another NIP (NOD26), and also in yeast plasma membranes which appear to have relatively high B permeability. We propose that tolerance to high soil B is mediated by reduced expression of HvNIP2;1 to limit B uptake as well as by increased expression of HvBot1 to remove B from roots and sensitive tissues. Together with HvBot1, the multifunctional aquaporin HvNIP2;1 is an important determinant of B toxicity tolerance in barley.
Keywords: Oocytes; Animals; Xenopus; Saccharomyces cerevisiae; Hordeum; Oryza sativa; Boron; Aquaporins; Plant Proteins; Chromosome Mapping; Cloning, Molecular; Gene Expression Regulation, Plant; Mutation; Quantitative Trait Loci; Models, Molecular; Molecular Sequence Data
Rights: Copyright © 2010 by the American Society of Plant Biologists
RMID: 0020100622
DOI: 10.1104/pp.110.158832
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Appears in Collections:Agriculture, Food and Wine publications

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