Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66155
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | MALDI Imaging Mass Spectrometry (MALDI-IMS): application of spatial proteomics for ovarian cancer classification and diagnosis |
Author: | Gustafsson, O. Oehler, M. Ruszkiewicz, A. McColl, S. Hoffmann, P. |
Citation: | International Journal of Molecular Sciences, 2011; 12(1):773-794 |
Publisher: | Molecular Diversity Preservation International (MDPI) AG. |
Issue Date: | 2011 |
ISSN: | 1422-0067 1422-0067 |
Statement of Responsibility: | Johan O. R. Gustafsson, Martin K. Oehler, Andrew Ruszkiewicz, Shaun R. McColl and Peter Hoffmann |
Abstract: | MALDI imaging mass spectrometry (MALDI-IMS) allows acquisition of mass data for metabolites, lipids, peptides and proteins directly from tissue sections. IMS is typically performed either as a multiple spot profiling experiment to generate tissue specific mass profiles, or a high resolution imaging experiment where relative spatial abundance for potentially hundreds of analytes across virtually any tissue section can be measured. Crucially, imaging can be achieved without prior knowledge of tissue composition and without the use of antibodies. In effect MALDI-IMS allows generation of molecular data which complement and expand upon the information provided by histology including immuno-histochemistry, making its application valuable to both cancer biomarker research and diagnostics. The current state of MALDI-IMS, key biological applications to ovarian cancer research and practical considerations for analysis of peptides and proteins on ovarian tissue are presented in this review. |
Keywords: | MALDI imaging mass spectrometry ovarian cancer grading biomarker |
Rights: | © 2011 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/). |
DOI: | 10.3390/ijms12010773 |
Published version: | http://dx.doi.org/10.3390/ijms12010773 |
Appears in Collections: | Aurora harvest IPAS publications Molecular and Biomedical Science publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_66155.pdf | Published version | 580.5 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.