Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/66330
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Type: | Journal article |
Title: | Extracellular matrix formation enhances the ability of streptococcus pneumoniae to cause invasive disease |
Author: | Trappetti, C. Ogunniyi, A. Oggioni, M. Paton, J. |
Citation: | PLoS One, 2011; 6(5):1-17 |
Publisher: | Public Library of Science |
Issue Date: | 2011 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Miyaji, E.N. |
Statement of Responsibility: | Claudia Trappetti, Abiodun D. Ogunniyi, Marco R. Oggioni and James C. Paton |
Abstract: | During infection, pneumococci exist mainly in sessile biofilms rather than in planktonic form, except during sepsis. However, relatively little is known about how biofilms contribute to pneumococcal pathogenesis. Here, we carried out a biofilm assay on opaque and transparent variants of a clinical serotype 19F strain WCH159. After 4 days incubation, scanning electron microscopy revealed that opaque biofilm bacteria produced an extracellular matrix, whereas the transparent variant did not. The opaque biofilm-derived bacteria translocated from the nasopharynx to the lungs and brain of mice, and showed 100- fold greater in vitro adherence to A549 cells than transparent bacteria. Microarray analysis of planktonic and sessile bacteria from transparent and opaque variants showed differential gene expression in two operons: the lic operon, which is involved in choline uptake, and in the two-component system, ciaRH. Mutants of these genes did not form an extracellular matrix, could not translocate from the nasopharynx to the lungs or the brain, and adhered poorly to A549 cells. We conclude that only the opaque phenotype is able to form extracellular matrix, and that the lic operon and ciaRH contribute to this process. We propose that during infection, extracellular matrix formation enhances the ability of pneumococci to cause invasive disease. |
Keywords: | Cell Line Extracellular Matrix Animals Humans Mice Biofilms Streptococcus pneumoniae Pneumococcal Infections Disease Models, Animal Bacterial Capsules Bacterial Proteins Blotting, Western Enzyme-Linked Immunosorbent Assay Biological Assay Colony Count, Microbial Administration, Intranasal Bacterial Adhesion Gene Expression Regulation, Bacterial Phenotype Mutation |
Description: | Extent: 17p. |
Rights: | © 2011 Trappetti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0019844 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0019844 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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hdl_66330.pdf | Published version | 1.88 MB | Adobe PDF | View/Open |
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