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https://hdl.handle.net/2440/66457
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Type: | Journal article |
Title: | Quality Control Guidelines for Amphotericin B, Itraconazole, Posaconazole, and Voriconazole Disk Diffusion Susceptibility Tests with Nonsupplemented Mueller-Hinton Agar (CLSI M51-A Document) for Nondermatophyte Filamentous Fungi |
Author: | Espinel-Ingroff, A. Canton, E. Fothergill, A. Ghannoum, M. Johnson, E. Jones, R. Ostrosky-Zeichner, L. Schell, W. Gibbs, D. Wang, A. Turnidge, J. |
Citation: | Journal of Clinical Microbiology, 2011; 49(7):2568-2571 |
Publisher: | Amer Soc Microbiology |
Issue Date: | 2011 |
ISSN: | 0095-1137 1098-660X |
Statement of Responsibility: | A. Espinel-Ingroff, E. Canton, A. Fothergill, M. Ghannoum, E. Johnson, R. N. Jones, L. Ostrosky-Zeichner, W. Schell, D. L. Gibbs, A. Wang and J. Turnidge |
Abstract: | Although Clinical and Laboratory Standards Institute (CLSI) disk diffusion assay standard conditions are available for susceptibility testing of filamentous fungi (molds) to antifungal agents, quality control (QC) disk diffusion zone diameter ranges have not been established. This multicenter study documented the reproducibility of tests for one isolate each of five molds (Paecilomyces variotii ATCC MYA-3630, Aspergillus fumigatus ATCC MYA-3626, A. flavus ATCC MYA-3631, A. terreus ATCC MYA-3633, and Fusarium verticillioides [moniliforme] ATCC MYA-3629) and Candida krusei ATCC 6258 by the CLSI disk diffusion method (M51-A document). The zone diameter ranges for selected QC isolates were as follows: P. variotii ATCC MYA-3630, amphotericin B (15 to 24 mm), itraconazole (20 to 31 mm), and posaconazole (33 to 43 mm); A. fumigatus ATCC MYA-3626, amphotericin B (18 to 25 mm), itraconazole (11 to 21 mm), posaconazole (28 to 35 mm), and voriconazole (25 to 33 mm); and C. krusei, amphotericin B (18 to 27 mm), itraconazole (18 to 26 mm), posaconazole (28 to 38 mm), and voriconazole (29 to 39 mm). Due to low testing reproducibility, zone diameter ranges were not proposed for the other three molds. |
Keywords: | Fungi Antifungal Agents Culture Media Microbial Sensitivity Tests Reproducibility of Results Mycology Quality Control |
Rights: | Copyright © 2011, American Society for Microbiology. All Rights Reserved. |
DOI: | 10.1128/JCM.00393-11 |
Published version: | http://dx.doi.org/10.1128/jcm.00393-11 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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