Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/69986
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | β-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-β-glucosidase bglD |
Other Titles: | beta-Glucoside metabolism in Oenococcus oeni: Cloning and characterisation of the phospho-beta-glucosidase bglD |
Author: | Capaldo, A. Walker, M. Ford, C. Jiranek, V. |
Citation: | Food Chemistry, 2011; 125(2):476-482 |
Publisher: | Elsevier Sci Ltd |
Issue Date: | 2011 |
ISSN: | 0308-8146 1873-7072 |
Statement of Responsibility: | A. Capaldo, M.E. Walker, C.M. Ford and V. Jiranek |
Abstract: | The structural gene for a phospho-β-glucosidase from the oenologically important lactic acid bacterium (LAB) Oenococcus oeni has been cloned and its protein product characterised. This gene is found in a putative β-glucosidase operon of 2178 base pairs encoding 4 genes designated bglA to bglD. The bglA, B and C genes were not cloned and characterised, however, are thought to be phosphoenolpyruvate dependent phospho transferase system (PEP-PTS) components IIC, IIA and IIB which regulate the uptake, phosphorylation and translocation of β-glucosides across the cytoplasmic membrane. The cloned bglD was sequenced and expressed in Escherichia coli followed by purification. The purified bglD protein has 480 residues, a molecular mass of 55.5kDa and shows high homology to known phospho-β-glucosidases. bglD exhibited high activity towards the phosphorylated β-glucoside para-nitrophenol-β-d-glucopyranoside-6-phosphate with a pH optimum of 5.5 and maintained similar levels of activity between temperatures of 4°C and 40°C. The enzyme was not active against non-phosphorylated β-glucosides. |
Keywords: | Phospho-b-glucosidase Oenococcus oeni Phosphoenolpyruvate-phospho transferase system |
Rights: | Copyright 2010 Elsevier Ltd. All rights reserved. |
DOI: | 10.1016/j.foodchem.2010.09.036 |
Published version: | http://dx.doi.org/10.1016/j.foodchem.2010.09.036 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.