Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/69996
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Highly parallel gene-to-BAC addressing using microarrays |
Author: | Liu, H. McNicol, J. Bayer, M. Morris, J. Cardle, L. Marshall, D. Schulte, D. Stein, N. Shi, B. Taudien, S. Waugh, R. Hedley, P. |
Citation: | BioTechniques: the journal of laboratory technology for bioresearch, 2011; 50(3):165-172 |
Publisher: | Eaton Publishing Co |
Issue Date: | 2011 |
ISSN: | 0736-6205 1940-9818 |
Statement of Responsibility: | Hui Liu, Jim McNicol, Micha Bayer, Jenny A. Morris, Linda Cardle, David F. Marshall, Daniela Schulte, Nils Stein, Bu-Jun Shi, Stefan Taudien, Robbie Waugh and Peter E. Hedley |
Abstract: | Second-generation sequencing now provides the potential for low-cost generation of whole-genome sequences. However, for large-genome organisms with high repetitive DNA content, genome-wide short read sequence assembly is currently impossible, with accurate ordering and localization of genes still relying heavily on integration with physical and genetic maps. To facilitate this process, we have used Agilent microarrays to simultaneously address thousands of gene sequences to individual BAC clones and contiguous sequences that form part of an emerging physical map of the large and currently unsequenced 5.3-Gb barley genome. The approach represents a cost-effective, highly parallel alternative to traditional addressing methods. By coupling the gene-to-BAC address data with gene-based molecular markers, thousands of BACs can be anchored directly to the genetic map, thereby generating a framework for orientating and ordering genes, and providing direct links to phenotypic traits. |
Keywords: | Chromosomes, Artificial, Bacterial Hordeum DNA, Plant Oligonucleotide Array Sequence Analysis Sequence Analysis, DNA Genome, Plant High-Throughput Nucleotide Sequencing |
Rights: | Copyright status unknown |
DOI: | 10.2144/000113627 |
Published version: | http://www.biotechniques.com/article/113627 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.