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https://hdl.handle.net/2440/73646
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Type: | Book chapter |
Title: | Comparative 2D DIGE analysis of the depleted serum proteome for biomarker discovery |
Author: | Penno, M. Ernst, M. Hoffmann, P. |
Citation: | Difference Gel Electrophoresis (DIGE): Methods and Protocols, 2012 / Cramer, R., Westermeier, R. (ed./s), vol.854, pp.207-220 |
Publisher: | Springer |
Publisher Place: | United States |
Issue Date: | 2012 |
ISBN: | 9781617795725 |
Editor: | Cramer, R. Westermeier, R. |
Statement of Responsibility: | Megan Penno, Matthias Ernst, and Peter Hoffmann |
Abstract: | Serum is unarguably the most used diagnostic fluid. As it circulates throughout the body, leakage peptides/proteins from damaged and dying cells, host-response proteins including inflammatory mediators, and aberrant secretions from tumors and diseased tissues are released into serum, potentially providing a rich source of disease biomarkers. Here, a method for extending access to the serum proteome by removing highly abundant proteins prior to comparative two-dimensional difference gel electrophoresis (2D DIGE) and subsequent protein digestion for identification by mass spectrometry is described. |
Keywords: | Biomarkers Serum Depletion Multiple affinity removal system Tryptic digestion |
Rights: | © Springer Science+Business Media, LLC 2012 |
DOI: | 10.1007/978-1-61779-573-2_15 |
Published version: | http://dx.doi.org/10.1007/978-1-61779-573-2_15 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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