Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/77480
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Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Curtin, C. | - |
dc.contributor.author | Borneman, A. | - |
dc.contributor.author | Chambers, P. | - |
dc.contributor.author | Pretorius, I. | - |
dc.contributor.editor | Fairhead, C. | - |
dc.date.issued | 2012 | - |
dc.identifier.citation | PLoS One, 2012; 7(3):1-10 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | http://hdl.handle.net/2440/77480 | - |
dc.description.abstract | Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits. | - |
dc.description.statementofresponsibility | Chris D. Curtin, Anthony R. Borneman, Paul J. Chambers and Isak S. Pretorius | - |
dc.language.iso | en | - |
dc.publisher | Public Library of Science | - |
dc.rights | © 2012 Curtin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | - |
dc.source.uri | http://dx.doi.org/10.1371/journal.pone.0033840 | - |
dc.subject | Sequence Analysis, DNA | - |
dc.subject | Phylogeny | - |
dc.subject | Haplotypes | - |
dc.subject | Polymorphism, Single Nucleotide | - |
dc.subject | Genome, Fungal | - |
dc.subject | Open Reading Frames | - |
dc.subject | Wine | - |
dc.subject | Dekkera | - |
dc.title | De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499 | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1371/journal.pone.0033840 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Borneman, A. [0000-0001-8491-7235] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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File | Description | Size | Format | |
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hdl_77480.pdf | Published version | 768.36 kB | Adobe PDF | View/Open |
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