Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/78351
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Trappetti, C. | - |
dc.contributor.author | van der Maten, E. | - |
dc.contributor.author | Amin, Z. | - |
dc.contributor.author | Potter, A. | - |
dc.contributor.author | Chen, A. | - |
dc.contributor.author | van Mourik, P. | - |
dc.contributor.author | Lawrence, A. | - |
dc.contributor.author | Paton, A. | - |
dc.contributor.author | Paton, J. | - |
dc.contributor.editor | Camilli, A. | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Infection and Immunity, 2013; 81(2):505-513 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/78351 | - |
dc.description.abstract | Streptococcus pneumoniae is a diverse species causing invasive as well as localized infections that result in massive global morbidity and mortality. Strains vary markedly in pathogenic potential, but the molecular basis is obscured by the diversity and plasticity of the pneumococcal genome. In the present study, S. pneumoniae serotype 3 blood (n = 12) or ear (n = 13) isolates were multilocus sequence typed (MLST) and assessed for biofilm formation and virulence phenotype. Blood and ear isolates exhibited similar MLST distributions but differed markedly in phenotype. Blood isolates formed robust biofilms only at pH 7.4, which were enhanced in Fe(III)-supplemented medium. Conversely, ear isolates formed biofilms only at pH 6.8, and Fe(III) was inhibitory. Biofilm formation paralleled luxS expression and genetic competence. In a mouse intranasal challenge model, blood isolates did not stably colonize the nasopharynx but spread to the blood; none spread to the ear. Ear isolates colonized the nasopharynx at higher levels and also spread to the ear compartment in a significant proportion of animals; none caused bacteremia. Thus, pneumococci of the same serotype and MLST exhibit distinct phenotypes in accordance with clinical site of isolation, indicative of stable niche adaptation within a clonal lineage. | - |
dc.description.statementofresponsibility | Claudia Trappetti, Erika van der Maten, Zarina Amin, Adam J. Potter, Austen Y. Chen, Paula M. van Mourik, Andrew J. Lawrence, Adrienne W. Paton, James C. Paton | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.rights | Copyright © 2013, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/iai.01033-12 | - |
dc.subject | Nasopharynx | - |
dc.subject | Animals | - |
dc.subject | Mice | - |
dc.subject | Biofilms | - |
dc.subject | Streptococcus pneumoniae | - |
dc.subject | Pneumococcal Infections | - |
dc.subject | Ferric Compounds | - |
dc.subject | Carbon-Sulfur Lyases | - |
dc.subject | Bacterial Proteins | - |
dc.subject | Bacterial Typing Techniques | - |
dc.subject | Serotyping | - |
dc.subject | Virulence | - |
dc.subject | Phenotype | - |
dc.subject | Genes, Bacterial | - |
dc.subject | Hydrogen-Ion Concentration | - |
dc.subject | Female | - |
dc.title | Site of isolation determines biofilm formation and virulence phenotypes of streptococcus pneumoniae serotype 3 clinical isolates | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/IAI.01033-12 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Trappetti, C. [0000-0001-8272-0068] | - |
dc.identifier.orcid | Amin, Z. [0000-0003-0513-7076] | - |
dc.identifier.orcid | Paton, J. [0000-0001-9807-5278] | - |
Appears in Collections: | Aurora harvest 4 Molecular and Biomedical Science publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.