Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/116147
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Type: | Journal article |
Title: | Real-time binding monitoring between human blood proteins and heavy metal ions in nanoporous anodic alumina photonic crystals |
Author: | Law, C. Lim, S. Abell, A. Santos, A. |
Citation: | Analytical Chemistry, 2018; 90(16):10039-10048 |
Publisher: | American Chemical Society |
Issue Date: | 2018 |
ISSN: | 0003-2700 1520-6882 |
Statement of Responsibility: | Cheryl Suwen Law, Siew Yee Lim, Andrew D. Abell, and Abel Santos |
Abstract: | This study reports on the real-time binding assessment between heavy metal ions and blood proteins immobilized onto nanoporous anodic alumina photonic crystals (NAA-PCs) by reflectometric interference spectroscopy (RIfS). The surface of NAA-PCs is chemically functionalized with γ-globulin (GG), transferrin (TFN), and serum albumin (HSA), the major proteins present in human blood plasma. Protein-modified NAA-PC platforms are exposed to analytical solutions of mercury ions of different concentrations. Dynamic changes in the effective optical thickness of protein-modified NAA-PCs in response to heavy metal ions are assessed in real time to evaluate the binding kinetics, affinity, and mechanism. Protein molecules undergo conformational changes upon exposure to mercury ions, with HSA exhibiting the strongest affinity. The combination of protein-modified NAA-PCs with RIfS allows real-time monitoring of protein-heavy metal ions interactions under dynamic flow conditions. This system is capable of detecting dynamic conformational changes in these proteins upon exposure to heavy metal ions. Our results provide new insights into these binding events, which could enable new methodologies to study the toxicity of heavy metal ions and other biomolecular interactions. |
Keywords: | Humans Aluminum Oxide Metals, Heavy Transferrin gamma-Globulins Spectrum Analysis Protein Conformation Protein Binding Porosity Serum Albumin, Human |
Rights: | © 2018 American Chemical Society |
DOI: | 10.1021/acs.analchem.8b02732 |
Grant ID: | http://purl.org/au-research/grants/arc/DE140100549 http://purl.org/au-research/grants/arc/CE140100003 |
Published version: | http://dx.doi.org/10.1021/acs.analchem.8b02732 |
Appears in Collections: | Aurora harvest 8 Chemical Engineering publications IPAS publications |
Files in This Item:
File | Description | Size | Format | |
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hdl_116147.pdf | Accepted version | 1.42 MB | Adobe PDF | View/Open |
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