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https://hdl.handle.net/2440/56836
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Type: | Journal article |
Title: | Channel-like characteristics of the low affinity barley phosphate transporter Pht1;6 when expressed in Xenopus Oocytes1,[W],[OA] |
Author: | Preuss, C. Huang, C. Gilliham, M. Tyerman, S. |
Citation: | Plant Physiology, 2010; 152(3):1431-1441 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2010 |
ISSN: | 0032-0889 1532-2548 |
Organisation: | Australian Centre for Plant Functional Genomics (ACPFG) |
Statement of Responsibility: | Christian P. Preuss, Chun Y. Huang, Matthew Gilliham, and Stephen D. Tyerman |
Abstract: | Remobilization of inorganic phosphate (Pi) within a plant is critical for sustaining growth and seed production under external Pi fluctuation. The barley (Hordeum vulgare) transporter HvPHT1;6 has been implicated in Pi remobilization. In this report, we expressed HvPHT1;6 in Xenopus laevis oocytes, allowing detailed characterization of voltage-dependent fluxes and currents induced by HvPHT1;6. HvPHT1;6 increased efflux of Pi near oocyte resting membrane potentials, dependent on external Pi concentration. Time-dependent inward currents were observed when membrane potentials were more negative than –160 mV, which was consistent with nH+:HPO42– (n > 2) cotransport, based on simultaneous radiotracer and oocyte voltage clamping, dependent upon Pi concentration gradient and pH. Time- and voltage-dependent inward currents through HvPHT1;6 were also observed for SO42–and to a lesser degree for NO3–Cl–but not for malate. Inward and outward currents showed linear dependence on the concentration of external HPO42–similar to low-affinity Pi transport in plant studies. The electrophysiological properties of HvPHT1;6, which locates to the plasma membrane when expressed in onion (Allium cepa) epidermal cells, are consistent with its suggested role in the remobilization of Pi in barley plants. |
Keywords: | Oocytes Animals Xenopus Hordeum Phosphates Phosphate Transport Proteins Plant Proteins DNA, Plant Cloning, Molecular Membrane Potentials |
Rights: | © 2010 American Society of Plant Biologists |
DOI: | 10.1104/pp.109.152009 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1104/pp.109.152009 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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