Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/77480
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Type: | Journal article |
Title: | De-novo assembly and analysis of the heterozygous triploid genome of the wine spoilage yeast Dekkera bruxellensis AWRI1499 |
Author: | Curtin, C. Borneman, A. Chambers, P. Pretorius, I. |
Citation: | PLoS One, 2012; 7(3):1-10 |
Publisher: | Public Library of Science |
Issue Date: | 2012 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Fairhead, C. |
Statement of Responsibility: | Chris D. Curtin, Anthony R. Borneman, Paul J. Chambers and Isak S. Pretorius |
Abstract: | Despite its industrial importance, the yeast species Dekkera (Brettanomyces) bruxellensis has remained poorly understood at the genetic level. In this study we describe whole genome sequencing and analysis for a prevalent wine spoilage strain, AWRI1499. The 12.7 Mb assembly, consisting of 324 contigs in 99 scaffolds (super-contigs) at 26-fold coverage, exhibits a relatively high density of single nucleotide polymorphisms (SNPs). Haplotype sampling for 1.2% of open reading frames suggested that the D. bruxellensis AWRI1499 genome is comprised of a moderately heterozygous diploid genome, in combination with a divergent haploid genome. Gene content analysis revealed enrichment in membrane proteins, particularly transporters, along with oxidoreductase enzymes. Availability of this assembly and annotation provides a resource for further investigation of genomic organization in this species, and functional characterization of genes that may confer important phenotypic traits. |
Keywords: | Sequence Analysis, DNA Phylogeny Haplotypes Polymorphism, Single Nucleotide Genome, Fungal Open Reading Frames Wine Dekkera |
Rights: | © 2012 Curtin et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0033840 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0033840 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 4 |
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hdl_77480.pdf | Published version | 768.36 kB | Adobe PDF | View/Open |
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