Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/90925
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Type: | Journal article |
Title: | Lipopolysaccharide surface structure does not influence IcsA polarity |
Author: | Doyle, M. Grabowicz, M. May, K. Morona, R. |
Citation: | FEMS Microbiology Letters, 2015; 362(8):fnv042-1-fnv042-7 |
Publisher: | Oxford University Press (OUP) |
Issue Date: | 2015 |
ISSN: | 0378-1097 1574-6968 |
Statement of Responsibility: | Matthew Thomas Doyle, Marcin Grabowicz, Kerrie Leanne May, and Renato Morona |
Abstract: | Shigella species are the causative agents of human bacillary dysentery. These bacteria spread within the lining of the gut via a process termed actin-based motility whereby an actin 'tail' is formed at the bacterial pole. The bacterial outer membrane protein IcsA initiates this process, and crucially is precisely positioned on the bacterial polar surface. Lipopolysaccharide (LPS) O-antigen surface structure has been implicated as an augmenting factor of polarity maintenance due to the apparent dysregulation of IcsA polarity in O-antigen deficient strains. Due to Shigellae having long and short O-antigen chains on their surfaces, it has been proposed that O-antigen chain lengths are asymmetrically distributed to optimize IcsA exposure at the pole and mask exposure laterally. Additionally, it has been proposed that LPS O-antigen restricts IcsA diffusion from the pole by maintaining minimal membrane fluidity. This study utilizes minicells and quantitative microscopy providing data refuting the models of asymmetric masking and membrane diffusion, and supporting a model of symmetric masking of IcsA. We contend that IcsA surface distribution is equivalent between wild-type and O-antigen deficient strains, and that differences in cellular IcsA levels have confounded previous conclusions. |
Keywords: | Shigella autotransporter bacterial pole lipopolysaccharide minicell outer membrane |
Rights: | © FEMS 2015. All rights reserved |
DOI: | 10.1093/femsle/fnv042 |
Grant ID: | http://purl.org/au-research/grants/nhmrc/565526 |
Published version: | http://dx.doi.org/10.1093/femsle/fnv042 |
Appears in Collections: | Aurora harvest 2 Molecular and Biomedical Science publications |
Files in This Item:
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hdl_90925.pdf | Accepted version | 1.31 MB | Adobe PDF | View/Open |
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